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A new enzymatic function in the melanogenic pathway. The 5,6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1).
TLDR
Results suggest that TRP1, the product of the brown locus, is indeed a tyrosinase with DHICA oxidase activity, and could be more directly related to DHICA metabolism than to the first steps of the pathway. Expand
Regulation of mammalian melanogenesis. I: Partial purification and characterization of a dopachrome converting factor: dopachrome tautomerase.
TLDR
The enzyme has the ability to increase the amount of melanin formed from L-tyrosine by melanoma tyrosinase, and therefore, cannot be considered an 'indole blocking factor' as was suggested for the related dopachrome oxidoreductase. Expand
Comparative action of dopachrome tautomerase and metal ions on the rearrangement of dopachrome.
TLDR
An improved background to look into the mode of action of DCT and metal ions is provided, enabling a clear cut differentiation between the effects of the two factors when both are present in biological extracts. Expand
Regulation of the final phase of mammalian melanogenesis. The role of dopachrome tautomerase and the ratio between 5,6-dihydroxyindole-2-carboxylic acid and 5,6-dihydroxyindole.
TLDR
It is suggested that DHI is essential for melanin formation, and the rate of polymerization depends on the ratio between DHICA and DHI in the medium. Expand
A new spectrophotometric assay for dopachrome tautomerase.
TLDR
A new procedure is presented that combines a quantitative, fast and easy way to prepare dopachrome from L-dopa by sodium periodate oxidation, and a spectrophotometric method in the UV region based on following the absorbance increase due to the enzyme-specific tautomerization of dopchrome to 5,6-dihydroxyindole-2-carboxylic acid. Expand
Molecular mechanism for catalysis by a new zinc-enzyme, dopachrome tautomerase.
TLDR
The results are consistent with Zn2+ chelation by the highly conserved histidine residues homologous to the histidines at the classical copper-binding sites in tyrosinase, which accounts for the reaction catalysed by DCT, i.e. a tautomerization, versus the copper-mediated oxidationsCatalysed by tyros in the mammalian eumelanogenic biosynthetic pathway. Expand
Regulation of mammalian melanogenesis. II: The role of metal cations.
TLDR
The study of the effects of some divalent metal ions (Zn, Cu, Ni and Co) on some steps of the melanogenesis pathway has been approached using different radiolabeled substrates and found the regulatory role of metal ions and dopachrome tautomerase does not seem to be mutually exclusive, but rather complementary. Expand
Tyrosinase isoenzymes in mammalian melanocytes. 1. Biochemical characterization of two melanosomal tyrosinases from B16 mouse melanoma.
TLDR
B-16 mouse melanoma melanosomes contain two forms of tyrosinase that can be resolved by SDS/PAGE and were stereospecific for the L isomers and sensitive to the specific tyrosine hydroxylase inhibitor 2-phenylthiourea. Expand
New insights on the structure of the mouse silver locus and on the function of the silver protein.
TLDR
Analysis of cDNA from the silver (si/si) melanocyte line, melan-si, showed that the pathogenic mutation is a G to A substitution at nt 1808, which yields a premature stop codon and a predicted protein truncated in the C-terminus, consistent with a stablin effect for the wild-type gp87 protein. Expand
The 5,6-dihydroxyindole-2-carboxylic acid (DHICA) oxidase activity of human tyrosinase.
TLDR
Comparison of the catalytic activities of human melanocytic cell lines expressing the full set of melanogenic enzymes or deficient in TYRP1, and transient expression of TYR and tyr genes in COS7 cells are used to demonstrate that human tyrosinase actually functions as a DHICA oxidase, as opposed to the mouse enzyme. Expand
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