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Cloning and Expression of a Fungal l-Arabinitol 4-Dehydrogenase Gene*
l-Arabinitol 4-dehydrogenase (EC 1.1.1.12) was purified from the filamentous fungusTrichoderma reesei (Hypocrea jecorina). It is an enzyme in the l-arabinose catabolic pathway of fungi catalyzing theExpand
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Drought tolerance in tobacco
Drought tolerance in tobacco Kjell-Ove Holmstrom;Einar Mantyla;Bjorn Welin;Abul Mandal;Tapio E. Palva;Outi E. Tunnela;John Londesborough; Nature
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Maltotriose utilization in lager yeast strains: MTT1 encodes a maltotriose transporter
Maltotriose is the second most abundant fermentable sugar in wort and, due to incomplete fermentation, residual maltotriose in beer causes both quality and economic problems in the brewing industry.Expand
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Engineering Redox Cofactor Regeneration for Improved Pentose Fermentation in Saccharomyces cerevisiae
ABSTRACT Pentose fermentation to ethanol with recombinant Saccharomyces cerevisiae is slow and has a low yield. A likely reason for this is that the catabolism of the pentoses d-xylose andExpand
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Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway.
The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose.Expand
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Maltose Transport by Brewer's Yeasts in Brewer's Wort
The kinetics of maltose transport by two industrial yeasts were studied. The ale and lager strain each showed both high and low affinity transport. For the lager strain, maltose transport was onlyExpand
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Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2.
We have isolated two genes from Saccharomyces cerevisiae that both encode a calmodulin-dependent protein kinase (CaM kinase). The CMK1 gene has been cloned by hybridization using an oligonucleotideExpand
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Identification of the first fungal NADP-GAPDH from Kluyveromyces lactis.
Deletion of the phosphoglucose isomerase gene, PGI1, in Saccharomyces cerevisiae leads to a phenotype for which glucose is toxic. This is related to overproduction of NADPH through the oxidative partExpand
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The temperature dependence of maltose transport in ale and lager strains of brewer's yeast
Lager beers are traditionally made at lower temperatures (6–14 °C) than ales (15–25 °C). At low temperatures, lager strains (Saccharomyces pastorianus) ferment faster than ale strains (SaccharomycesExpand
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The missing link in the fungal L-arabinose catabolic pathway, identification of the L-xylulose reductase gene.
The fungal L-arabinose pathway consists of five enzymes, aldose reductase, L-arabinitol 4-dehydrogenase, L-xylulose reductase, xylitol dehydrogenase, and xylulokinase. All the genes encoding theExpand
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