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Cholesterol Is Superior to 7-Ketocholesterol or 7α-Hydroxycholesterol as an Allosteric Activator for Acyl-coenzyme A:Cholesterol Acyltransferase 1*
We compared the abilities of cholesterolversus various oxysterols as substrate and/or as activator for the enzyme acyl-coenzyme A:cholesterol acyltransferase (ACAT), by monitoring the activity ofExpand
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Investigating the allosterism of acyl-CoA:cholesterol acyltransferase (ACAT) by using various sterols: in vitro and intact cell studies.
ACAT1 (acyl-CoA:cholesterol acyltransferase 1) is thought to have two distinct sterol-binding sites: a substrate-binding site and an allosteric-activator site. In the present work, we investigatedExpand
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  • Open Access
Erv41p and Erv46p
Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicleExpand
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  • Open Access
Human acyl-CoA:cholesterol acyltransferase (ACAT) and its potential as a target for pharmaceutical intervention against atherosclerosis.
Acyl-CoA:cholesterol acyltransferase (ACAT) catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A. At the single-cell level, ACAT serves as a regulatorExpand
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Quantification of protein mixture in chromatographic separation using multi‐wavelength UV spectra
In therapeutic protein production, the protein purification with chromatographic processes is of high importance in separating the qualified proteins from the impurities for consistent productExpand
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Human Acyl-CoA:Cholesterol Acyltransferase-1 Is a Homotetrameric Enzyme in Intact Cells and in Vitro *
Acyl-CoA:cholesterol acyltransferase (ACAT) is a key enzyme in cellular cholesterol homeostasis and in atherosclerosis. ACAT-1 may function as an allosteric enzyme. We took a multifaceted approach toExpand
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  • Open Access
Cellular Pregnenolone Esterification by Acyl-CoA:Cholesterol Acyltransferase*
Background: Various sterols serve as substrates and allosteric activators for ACAT. Results: Pregnelonone is the only natural sterol that is an ACAT substrate but not an ACAT activator. Conclusion:Expand
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  • Open Access
Multiparameter exploration of piperazine derivatives as δ-opioid receptor agonists for CNS indications.
A novel series of piperazine derivatives exhibits sub-nanomolar binding and enhanced subtype selectivity as δ-opioid agonists. The synthesis and SAR are described as well as the application ofExpand
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4-Piperidin-4-ylidenemethyl-benzamides as δ-opioid receptor agonists for CNS indications: identifying clinical candidates.
A series of 4-piperidin-4-ylidenemethyl-benzamide δ-opioid receptor agonists is described with an emphasis on balancing the potency, subtype selectivity and in vitro ADME and safety properties. TheExpand
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Substrate inhibition of fibrin-dependent plasminogen activation by tissue-type plasminogen activator.
  • J. Liu, V. Gurewich
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 15 June 1993
Tissue plasminogen activator (t-PA) is a poor activator of plasminogen, but its catalytic efficiency is greatly enhanced in the presence of fibrin or certain fibrin derivatives. These two veryExpand
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