• Publications
  • Influence
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
TLDR
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
We report a photoactivatable variant of theAequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by
Brefeldin A: insights into the control of membrane traffic and organelle structure
TLDR
The relationship between the control of membrane traffic and the maintenance of organelle structure has been investigated with the use of a remarkable drug, brefeldin A (BFA), and some speculative models concerning the mechanism and regulation ofmembrane traffic within the central vacuolar system are proposed.
ER-to-Golgi transport visualized in living cells
TLDR
Results indicate that these pre-Golgi carrier structures moving unidirectionally along microtubule tracks are responsible for transporting VSVG-GFP through the cytoplasm to the Golgi complex, which contrasts with the traditional focus on small vesicles as the primary vehicles for ER-to-golgi transport.
Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis
TLDR
Selective changes in lateral mobility of LBR–GFP within the ER/NE membrane system form the basis for its localization to the inner nuclear membrane during interphase, and also underlie the redistribution of this molecule during NE disassembly and reformation in mitosis.
Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes
TLDR
The mammalian homologue of Atg9p, a multi-spanning transmembrane protein essential in yeast for autophagy, is studied to gain a better understanding of the function of this ubiquitious protein.
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
TLDR
A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
Studying protein dynamics in living cells
TLDR
Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components.
Maintenance of Golgi structure and function depends on the integrity of ER export
TLDR
The results suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1–COPII and Arf1–coatomer systems.
...
...