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Post‐transfer editing in vitro and in vivo by the β subunit of phenylalanyl‐tRNA synthetase
Translation of the genetic code requires attachment of tRNAs to their cognate amino acids. Errors during amino‐acid activation and tRNA esterification are corrected by aminoacyl‐tRNAExpand
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Severe oxidative stress induces protein mistranslation through impairment of an aminoacyl-tRNA synthetase editing site
  • J. Ling, D. Söll
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 16 February 2010
Oxidative stress arises from excessive reactive oxygen species (ROS) and affects organisms of all three domains of life. Here we present a previously unknown pathway through which ROS may impactExpand
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Aminoacyl-tRNA synthesis and translational quality control.
Translating the 4-letter code of RNA into the 22-letter alphabet of proteins is a central feature of cellular life. The fidelity with which mRNA is translated during protein synthesis is determinedExpand
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Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures.
Progressive microcephaly is a heterogeneous condition with causes including mutations in genes encoding regulators of neuronal survival. Here, we report the identification of mutations in QARSExpand
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Loss of Editing Activity during the Evolution of Mitochondrial Phenylalanyl-tRNA Synthetase*
Accurate selection of amino acids is essential for faithful translation of the genetic code. Errors during amino acid selection are usually corrected by the editing activity of aminoacyl-tRNAExpand
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Upgrading protein synthesis for synthetic biology.
Genetic code expansion for synthesis of proteins containing noncanonical amino acids is a rapidly growing field in synthetic biology. Creating optimal orthogonal translation systems will requireExpand
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Mechanism of tRNA-dependent editing in translational quality control
  • J. Ling, H. Roy, M. Ibba
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 2 January 2007
Protein synthesis requires the pairing of amino acids with tRNAs catalyzed by the aminoacyl-tRNA synthetases. The synthetases are highly specific, but errors in amino acid selection are occasionallyExpand
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Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria
The discovery of diverse codon reassignment events has demonstrated that the canonical genetic code is not universal. Studying coding reassignment at the molecular level is critical for understandingExpand
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An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine
The standard genetic code is used by most living organisms, yet deviations have been observed in many genomes, suggesting that the genetic code has been evolving. In certain yeast mitochondria, CUNExpand
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Cell-specific differences in the requirements for translation quality control
Protein synthesis has an overall error rate of approximately 10-4 for each mRNA codon translated. The fidelity of translation is mainly determined by two events: synthesis of cognate amino acid:tRNAExpand
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