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Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
TLDR
Each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene), for example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglions or only layer 5 cortical pyramids were labeled in others. Expand
Development of the vertebrate neuromuscular junction.
TLDR
The extent to which the NMJ is a suitable model for development of neuron-neuron synapses is considered, and an additional set of cues biases synapse formation in favor of appropriate partners. Expand
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
TLDR
Strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours are presented and may facilitate the analysis of neuronal circuitry on a large scale. Expand
Induction, assembly, maturation and maintenance of a postsynaptic apparatus
TLDR
The molecular and cellular mechanisms that govern each of these stages are now being elucidated by a combination of microscopic and genetic methods, allowing the neuromuscular junction to serve as a model for smaller and less-accessible central synapses. Expand
Saturated Reconstruction of a Volume of Neocortex
We describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which allExpand
In vivo imaging of axonal degeneration and regeneration in the injured spinal cord
TLDR
Monitoring individual fluorescent axons in the spinal cords of living transgenic mice over several days after spinal injury suggests that time-lapse imaging of spinal Cord injury may provide a powerful analytical tool for assessing the pathogenesis of spinal cord injury and for evaluating therapies that enhance regeneration. Expand
Imaging axonal transport of mitochondria in vivo
TLDR
It is shown that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport and in vivo imaging of mitochondria will be a useful tool to analyze this essential organelle. Expand
Clarifying Tissue Clearing
TLDR
The physical basis for light scatter in tissue is introduced, the mechanisms underlying various clearing techniques are described, and several of the major advances in light microscopy for imaging cleared tissue are discussed. Expand
Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise
TLDR
A critical effect of aging on synaptic structure is demonstrated and evidence that interventions capable of extending health span and lifespan can partially reverse these age-related synaptic changes is provided. Expand
Improved tools for the Brainbow toolbox.
TLDR
This work presents several lines of mice that overcome limitations of the initial lines, and reports an adaptation of the method for use in adeno-associated viral vectors, and provides technical advice about how best to image Brainbow-expressing tissue. Expand
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