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Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Development of the vertebrate neuromuscular junction.
The extent to which the NMJ is a suitable model for development of neuron-neuron synapses is considered, and an additional set of cues biases synapse formation in favor of appropriate partners.
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours are presented and may facilitate the analysis of neuronal circuitry on a large scale.
Induction, assembly, maturation and maintenance of a postsynaptic apparatus
The molecular and cellular mechanisms that govern each of these stages are now being elucidated by a combination of microscopic and genetic methods, allowing the neuromuscular junction to serve as a model for smaller and less-accessible central synapses.
Saturated Reconstruction of a Volume of Neocortex
Clarifying Tissue Clearing
In vivo imaging of axonal degeneration and regeneration in the injured spinal cord
Monitoring individual fluorescent axons in the spinal cords of living transgenic mice over several days after spinal injury suggests that time-lapse imaging of spinal Cord injury may provide a powerful analytical tool for assessing the pathogenesis of spinal cord injury and for evaluating therapies that enhance regeneration.
Imaging axonal transport of mitochondria in vivo
It is shown that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport and in vivo imaging of mitochondria will be a useful tool to analyze this essential organelle.
Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise
- Gregorio Valdez, J. Tapia, J. Sanes
- BiologyProceedings of the National Academy of Sciences
- 2 August 2010
A critical effect of aging on synaptic structure is demonstrated and evidence that interventions capable of extending health span and lifespan can partially reverse these age-related synaptic changes is provided.
Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition
It is shown that more powerful inputs are strongly favoured competitors during synapse elimination, and a genetic method is used to selectively inhibit neurotransmission from one of two inputs to a single target cell.