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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
This strategy uses the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis to achieve high-yield, high-specificity production of isobutanol from glucose.
Network component analysis: Reconstruction of regulatory signals in biological systems
- J. Liao, R. Boscolo, Young-Lyeol Yang, L. Tran, C. Sabatti, V. Roychowdhury
- Medicine, Computer ScienceProceedings of the National Academy of Sciences…
- 12 December 2003
This work develops a method, called network component analysis, for uncovering hidden regulatory signals from outputs of networked systems, when only a partial knowledge of the underlying network topology is available.
Metabolic engineering of Escherichia coli for 1-butanol production.
A synthetic pathway is engineered in Escherichia coli and the production of 1-butanol is demonstrated from this non-native user-friendly host, showing promise for using E. coli for 1- butanol production.
Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
- Claire R. Shen, Ethan I Lan, Y. Dekishima, Antonino Baez, K. Cho, J. Liao
- Medicine, BiologyApplied and Environmental Microbiology
- 11 March 2011
A modified clostridial 1-butanol pathway is constructed in Escherichia coli to provide an irreversible reaction catalyzed by trans-enoyl-coenzyme A (CoA) reductase (Ter) and NADH and acetyl-CoA driving forces to direct the flux and demonstrate the importance of driving forces in the efficient production of nonnative products.
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
The genetically engineered Synechococcus elongatus PCC7942 strain is genetically engineered to produce isobutyraldehyde and isobutanol directly from CO2 and increased productivity by overexpression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco).
Issues in cDNA microarray analysis: quality filtering, channel normalization, models of variations and assessment of gene effects.
A quality index, computed from duplicate spots on the same slide, is used to filter out outlying spots, poor quality genes and problematical slides and a rank invariant method is suggested to select non-differentially expressed genes and to construct normalization curves in comparative experiments.
Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways.
This work systematically improved the synthesis of 1-propanol and 1-butanol through deregulation of amino-acid biosynthesis and elimination of competing pathways.
Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide.
This result represents the first autotrophic 1-butanol production from CO(2) from a cyanobacterium and demonstrates the activity of each enzyme in the pathway by chromosomal integration and expression of the genes.
Global Expression Profiling of Acetate-grown Escherichia coli *
- M. Oh, L. Rohlin, Katy C. Kao, J. Liao
- Biology, MedicineThe Journal of Biological Chemistry
- 12 April 2002
The gene expression profiles qualitatively agree with the metabolic flux changes and may serve as a predictor for gene function and metabolic flux distribution.
An integrated network approach identifies the isobutanol response network of Escherichia coli
The network described here could aid design and comprehension of alcohol tolerance, whereas the approach provides a general framework to characterize complex phenomena at the systems level.