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One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies
ABSTRACT We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning canExpand
The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism.
Members of the genus Thermococcus, sulfur-reducing hyperthermophilic archaea, are ubiquitously present in various deep-sea hydrothermal vent systems and are considered to play a significant role inExpand
Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide.
A bacterium (named OT-1(T)) that showed algicidal activity was isolated from sea water of Masan Bay, Korea, during an outbreak of red tide. Phylogenetic analysis based on 16S rDNA sequences showedExpand
Physical characteristics and age structure of Mongolian racerunner (Eremias argus; Larcertidae; Reptilia)
In this study, we have evaluated the physical characteristics of neonate, female, and male Mongolian racerunners (Er- emias argus) and determined the age structure of a population of the species in aExpand
Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment
To search for novel lipolytic enzymes, a metagenomic library was constructed from the tidal flat sediment of Ganghwa Island in South Korea. By functional screening using tributyrin agar plates, 3Expand
Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga
Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026(T) and MEBiC 08903(T), were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNAExpand
Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome
To search for new cold-active lipases, a metagenomic library was constructed using cold-sea sediment samples at Edison Seamount and was screened for lipolytic activities by plating on a tricaprylinExpand
Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456.
A soluble Cr(VI) reductase was purified from the cytoplasm of Escherichia coli ATCC 33456. The molecular mass was estimated to be 84 and 42 kDa by gel filtration and SDS-polyacrylamide gelExpand
Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome.
A metagenomics approach is an efficient method of isolating novel and useful genes from uncultured microorganisms in diverse environments. In this research, a gene encoding a new esterase (EM2L8) wasExpand
Characterization and its Potential Application of Two Esterases Derived from the Arctic Sediment Metagenome
Two esterase genes (designated as estAT1 and estAT11, respectively) were cloned by activity-based screening of a fosmid library constructed with seashore sediment sample of the Arctic. The sequenceExpand