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Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung.
Growth of P. aeruginosa in artificial sputum medium is an appropriate model of chronic lung colonization and may be useful for evaluating therapeutic procedures and studying antibiotic-resistance mechanisms. Expand
Genetics of O-Antigen Biosynthesis inPseudomonas aeruginosa
P. aeruginosa represents a unique model system, allowing studies of heteropolymeric and homopolymeric O-antigen synthesis, as well as permitting an examination of the interrelationship of the synthesis of LPS molecules and other virulence determinants. Expand
Review: Lipopolysaccharide biosynthesis in Pseudomonas aeruginosa
A full understanding of LPS biosynthesis in P. aeruginosa is almost within reach, and knowledge derived from detailed studies in the O5, O6 and O11 serotypes is applied to predict biosynthesis pathways of sugars in poorly-studied serotypes. Expand
Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis
Pseudomonas aeruginosa appear to evolve during chronic colonization in CF patients from specific adaptation to infection rather than from acquisition of new bacterial strains. Expand
Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di‐rhamnolipid biosynthesis
The genetic regulation of rhlC by RpoN and RhlR was in agreement with the observed increasing RhlC rhamnosyltransferase activity during the stationary phase of growth, the first report of a rhamanosyl transferase gene responsible for the biosynthesis of di‐rhamnolipid in P. aeruginosa. Expand
Genetic and Functional Diversity of Pseudomonas aeruginosa Lipopolysaccharide
The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa and its contributions to host–pathogen interactions and the control/prevention of infection. Expand
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production
It is revealed that 11 psl genes, pslACDEFGHIJKL, are required for Psl production and surface attachment and the first structural analysis of the psl‐dependent polysaccharide, which consists of a repeating pentasaccharide containing d‐mannose, d‐glucose and l‐rhamnose. Expand
The structural basis of the catalytic mechanism and regulation of glucose‐1‐phosphate thymidylyltransferase (RmlA)
The structures of five distinct enzyme substrate–product complexes reveal the enzyme mechanism that involves precise positioning of the nucleophile and activation of the electrophile, suggesting that the basic mechanism is found in many nucleotidyltransferases. Expand
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide.
This study reports the organization of the wbp gene cluster and characterization of a number of genes that are essential for B-band O antigen biosynthesis in the clinically prevalent PseudomonasExpand
Synthesis of bacterial polysaccharides via the Wzx/Wzy-dependent pathway.
This review has provided a comprehensive synthesis of the latest structural and mechanistic data for each protein, as well as an examination of substrate specificity for each assembly step and complex formation between the constituent proteins. Expand