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Principles of fluorescence spectroscopy
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of theExpand
Principles of Fluorescence Spectroscopy, Third Edition
This PDF file contains the editorial “Principles of Fluorescence Spectroscopy, Third Edition” for JBO Vol. 13 Issue 02
Radiative decay engineering: biophysical and biomedical applications.
  • J. Lakowicz
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1 November 2001
TLDR
This forward-looking article describes a new opportunity in fluorescence, radiative decay engineering (RDE), and predicts that nearby metal surfaces can be used to increase the low intrinsic quantum yields of nucleic acids and make unlabeled DNA detectable using its intrinsic metal-enhanced fluorescence. Expand
Fluorescence lifetime imaging of free and protein-bound NADH.
TLDR
FLIM is used to create lifetime images of NADH when free in solution and when bound to malate dehydrogenase, and describes am imaging procedure that allows specific decay times to be suppressed, allowing in this case suppression of the emission from either free or bound NADH. Expand
Fluorescence lifetime imaging.
We describe a new fluorescence imaging methodology in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentrationExpand
Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission.
  • J. Lakowicz
  • Chemistry, Medicine
  • Analytical biochemistry
  • 15 February 2005
TLDR
The RP model provides a rational approach for designing fluorophore-metal configurations with the desired emissive properties and a basis for nanophotonic fluorophores technology. Expand
Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale.
TLDR
Quenching of the tryptophan fluorescence of proteins was studied using oxygen concentrations up to 0.13 M, corresponding to equilibration with oxygen at a pres- sure of 1500 psi, and revealed no significant perturbation of the protein structure. Expand
Quenching of Fluorescence
Fluorescence quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching. These include excited state reactions,Expand
Topics in fluorescence spectroscopy
Topics in fluorescence spectroscopy , Topics in fluorescence spectroscopy , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
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