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Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
TLDR
The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities, and a checklist is provided with summaries of key points.
View on Taylor & Francis
Biological properties of extracellular vesicles and their physiological functions
TLDR
A comprehensive overview of the current understanding of the physiological roles of EVs is provided, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia.
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Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles
TLDR
The International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.
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Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
TLDR
The need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results is emphasized, and it is recognized that continual development and evaluation of techniques will be necessary as new knowledge is amassed.
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Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation
TLDR
Vesiclepedia is a community-annotated compendium of molecular data on extracellular vesicles that aims to provide a single authoritative source for information on vesicle structure and function.
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Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways.
TLDR
It is demonstrated that hIL-17 can specifically and selectively recruit neutrophils into the airways via the release of C-X-C chemokines from bronchial epithelial cells and suggest a novel mechanism linking the activation of T-lymphocytes to recruitment of neutrophil into theAirways.
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Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes
TLDR
The authors' results demonstrate that centrifugation-based protocols are simple and fast systems to distinguish subpopulations of extracellular vesicles, but they are indistinguishable using CD63-coated beads for flow cytometry analysis.
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Brain metastatic cancer cells release microRNA-181c-containing extracellular vesicles capable of destructing blood–brain barrier
TLDR
It is shown that cancer-derived extracellular vesicles, mediators of cell–cell communication via delivery of proteins and microRNAs (miRNAs), trigger the breakdown of BBB, and that systemic injection of brain metastatic cancer cell-derived EVs promoted brain metastasis of breast cancer cell lines and are preferentially incorporated into the brain in vivo.
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Asthma endotypes: a new approach to classification of disease entities within the asthma syndrome.
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