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Enhanced degradation of polycyclic aromatic hydrocarbons by biodegradation combined with a modified Fenton reaction.
TLDR
By the combined treatment of the modified Fenton reaction and biodegradation, more than 98% of 2- or 3-ring hydrocarbons and between 70% and 85% of 4- or 5-ring compounds were degraded in the MGP soil, while maintaining its pH about 6-6.5. Expand
Diversity of benzyl- and alkylsuccinate synthase genes in hydrocarbon-impacted environments and enrichment cultures.
TLDR
Data confirm that the assA gene is a useful biomarker for anaerobic alkane metabolism and expand the range of alkane-degrading conditions for which relevant gene sequences are available and indicate that considerable diversity of assA genes can be found in hydrocarbon-impacted environments. Expand
A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1
TLDR
Plasmid pRO1957, which contains a 26.5-kb fragment from the chromosome of Pseudomonas pickettii PKO1, allows P. aeruginosa PAO1 to grow on toluene or benzene as a sole carbon and energy source, suggests that toLUene-3-monooxygenation is the first step in the degradative pathway. Expand
Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments
TLDR
Strain PKO1 compensates for a low-oxygen environment by the development of an oxygen-requiring enzyme with kinetic parameters favorable to function in hypoxic environments, as well as by elevating synthesis of such an enzyme in response to oxygen limitation. Expand
Microbial Dioxygenase Gene Population Shifts during Polycyclic Aromatic Hydrocarbon Biodegradation
TLDR
Molecular monitoring of the enrichment cultures before and after PAH degradation using denaturing gradient gel electrophoresis and 16S rRNA gene libraries suggests that specific phylotypes of bacteria were associated with the degradation of each PAH. Expand
The genome sequence of Desulfatibacillum alkenivorans AK-01: a blueprint for anaerobic alkane oxidation.
TLDR
Genomic analysis suggested a route to regenerate the fumarate needed for alkane activation via methylmalonyl-CoA and predicted the capability for syntrophic alkane metabolism, which was experimentally verified. Expand
Identification of Unique Type II Polyketide Synthase Genes in Soil
TLDR
To directly access PKS gene diversity from soil, degenerate PCR primers for actinomycete type II KSα (ketosynthase) genes were developed and one cluster of sequences was most similar to the KSα involved in ardacin (glycopeptide antibiotic) production by Kibdelosporangium aridum. Expand
Characterization and Role of tbuX in Utilization of Toluene by Ralstonia pickettii PKO1
TLDR
Based on sequence analysis, transcriptional and expression studies, and deletion analysis, TbuX seems to play an important role in the catabolism of toluene in R. pickettii PKO1 and appears to be regulated in a manner such that low levels of tbuX are always present within the cell, whereas upon toluenes exposure these levels dramatically increase, even more than those of toLUene-3-monooxygenase. Expand
Regulation of tfdCDEF by tfdR of the 2,4-dichlorophenoxyacetic acid degradation plasmid pJP4
TLDR
Derepression of tfdC was achieved when Pseudomonas aeruginosa PAO4032 containing both plasmids pRO2334 and pRO1949 was grown in minimal glucose medium containing TFD, 2,4-dichlorophenol, or 4-chlorocatechol, suggesting that TFD and other pathway intermediates can act as inducing compounds. Expand
Genetic organization and regulation of a meta cleavage pathway for catechols produced from catabolism of toluene, benzene, phenol, and cresols by Pseudomonas pickettii PKO1
TLDR
Meta pathway enzymes were repressed in the absence of an effector and were fully induced when an effectors was present, suggesting that the gene product of tbuS acts as both a repressor and an activator in meta pathway enzymatic activity. Expand
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