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Characterization of Encapsulated and Noncapsulated Haemophilus influenzae and Determination of Phylogenetic Relationships by Multilocus Sequence Typing
A multilocus sequence typing (MLST) scheme has been developed for the unambiguous characterization of encapsulated and noncapsulated Haemophilus influenzae isolates, finding significant congruence between many of the trees reconstructed from the sequences of the seven individual loci. Expand
PCR for capsular typing of Haemophilus influenzae
PCR capsular typing overcomes the problems of cross-reaction and autoagglutination associated with the serotyping of H. influenzae strains. Expand
The Haemophilus influenzae capsulation gene cluster: a compound transposon
It is shown that in division I strains the capsulation (cap) gene cluster lies between direct repeats of a novel insertion sequence (IS)‐like element, IS 1016, which makes capsulation dependent on preservation of the direct repeat structure in order to avoid recombination‐mediated loss of the other copy of bexA. Expand
The role of bacterial polysaccharide capsules as virulence factors.
  • E. Moxon, J. Kroll
  • Biology, Medicine
  • Current topics in microbiology and immunology
  • 1990
Among certain gram-positive and gram-negative bacteria, capsules have evolved distinctive structural and functional characteristics which are of cardinal importance in the pathogenesis of infections of animals, plants and insects. Expand
The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export
Comparison of the deduced products of bexC, bexB, and bexA to known proteins, and TnphoA mutagenesis, suggests that they form components of an ATP‐driven polysaccharide export apparatus. Expand
Natural genetic transfer of a putative virulence-enhancing mutation to Haemophilus influenzae type a.
Examination of strains from the Gambia, West Africa, that caused septicemia, meningitis, or both revealed that a clone has emerged that carries a DNA deletion previously identified only in type b strains that is hypothesized to contribute to the special virulence of that serotype. Expand
Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae.
The analysis suggests that the present distribution of clones is, in part, related to patterns of racial or ethnic differentiation and historical demographic movements of the human host populations. Expand
Capsule loss in H. influenzae type b occurs by recombination-mediated disruption of a gene essential for polysaccharide export
It is shown that capsular polysaccharide export might occur via an energy-dependent transporter with similarities to those identified for the import of various substrates into Gram-negative bacteria, BexA being the "energizer" of the transporter. Expand
Clonal population structure of encapsulated Haemophilus influenzae
The occurrence of strong associations of characters and the recovery of isolates with identical genetic properties in widely separated geographic regions and over a 40-year period indicated that the population structure of encapsulated H. influenzae is clonal. Expand
Region II of the Haemophilus influenzae Type b capsulation locus as involved in serotype‐specific polysaccharide synthesis
It is presented evidence that growth of polysaccharide chains takes place through the alternating addition of single sugar nucleotides and that orf1 probably encodes a CDP‐ribitolpyrophosphorylase. Expand