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Lymphocyte proliferation is controlled by both iron availability and regulation of iron uptake pathways.
Differences in sensitivity to the effects of these agents are influenced by the amount of available transferrin-Fe and differences in the mechanisms that individual lymphocyte cell lines utilize to ensure adequate Fe uptake to support proliferation. Expand
Reduction of quaternary benzophenanthridine alkaloids by NADH and NADPH
A sensitive photometric assay for alcohol dehydrogenase activity in blood serum.
Minutes activities of alcohol dehydrogenase in blood serum can be determined from the fading of the yellow color of p-nitrosodimethylaniline in a recycling reaction, which shows the activity of this aldehyde-like substrate. Expand
Serum-free medium for hybridoma and parental myeloma cell cultivation.
Differences in the interactions of liver alcohol dehydrogenases with probes binding into the substrate pocket.
The data presented confirm the existence of three distinct binding sites for these ligands in the substrate pocket of liver alcohol dehydrogenase, which are moderately hydrophobic and that for acridines is rather polar. Expand
Interaction of liver alcohol dehydrogenase with protoberberine alkaloids
Characterization of binding site of horse liver alcoholdehydrogenase for berberines and auramine O
Growth-stimulating effect of ferric citrate on hybridoma cells: characterization and relation to transferrin function.
- J. Kovar
- Biology, Medicine
- 1 June 1988
Experiments with monoclonal antibody to mouse transferrin receptor demonstrate that the growth-stimulating effect of ferric citrate is not based on the action of residual transferrin or theaction of transferrin secreted by hybridoma cells, specific for iron compounds while other metal trace elements are ineffective. Expand
INHIBITION OF LADH BY BERBERINE AND SOME RELATED ALKALOIDS
SUMMARY The alkaloid berberine inhibits oxidation of ethanol by LADH competitively both towards the substrate and the coenzyme NAD + . Fluorometric and spectrophotometry equilibrium measurements… Expand
The inability of cells to grow in low iron correlates with increasing activity of their iron regulatory protein (IRP)
- J. Kovar, L. Kühn, V. Richardson, C. Seiser, K. Kriegerbecková, J. Musilkova
- Biology, Medicine
- In Vitro Cellular & Developmental Biology…
- 1 September 1997
It is hypothesized that the relatively higher bioavailability of nontransferrin iron in LIT cells, over that in HID cells, determines the differing responses observed under low-iron conditions. Expand