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Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay.
The kinetic properties and substrate specificity of two well-characterized peptidyl prolyl cis-trans isomerases (PPIases), cyclophilin and the FK-506 binding protein (FKBP), have been previouslyExpand
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Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway.
A small molecule, alpha-(trichloromethyl)-4-pyridineethanol (PETCM), was identified by high-throughput screening as an activator of caspase-3 in extracts of a panel of cancer cells. PETCM was used inExpand
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Do structurally similar molecules have similar biological activity?
To design diverse combinatorial libraries or to select diverse compounds to augment a screening collection, computational chemists frequently reject compounds that are > or =0.85 similar to oneExpand
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Distinctive Roles of PHAP Proteins and Prothymosin-α in a Death Regulatory Pathway
A small molecule, α-(trichloromethyl)-4-pyridineethanol (PETCM), was identified by high-throughput screening as an activator of caspase-3 in extracts of a panel of cancer cells. PETCM was used inExpand
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Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay.
The kinetic properties and substrate specificity of two well-characterized peptidyl prolyl cis-trans isomerases (PPIases), cyclophilin and the FK-506 binding protein (FKBP), have been previouslyExpand
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Lithium chloride perturbation of cis-trans peptide bond equilibria: effect on conformational equilibria in cyclosporin A and on time-dependent inhibition of cyclophilin
The cyclic undecapeptide cyclosporin A (CsA) is a slow-binding inhibitor of the peptidylprolyl cis-trans isomerase (PPIase) cyclophiiin. Both the initial inhibitory activity and the subsequentExpand
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Identification of actin and HSP 70 as cyclosporin A binding proteins by photoaffinity labeling and fluorescence displacement assays.
A novel family of cyclosporin A (CsA) binding proteins was identified by using the biologically active, radioiodinated photoaffinity probe [D-Lys-NExpand
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Microarrayed Compound Screening (μARCS) to Identify Activators and Inhibitors of AMP-Activated Protein Kinase
A novel and innovative high-throughput screening assay was developed to identify both activators and inhibitors of AMP-activated protein kinase (AMPK) using microarrayed compound screening (μARCS)Expand
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Microarray Compound Screening (μARCS) to Identify Inhibitors of HIV Integrase
A novel high-throughput strand transfer assay has been developed, using Microarray Compound Screening (μARCS) technology, to identify inhibitors of human immunodeficiency virus (HIV) integrase. ThisExpand
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Substrate activity of synthetic formyl phosphate in the reaction catalyzed by formyltetrahydrofolate synthetase.
Formyl phosphate, a putative enzyme-bound intermediate in the reaction catalyzed by formyltetrahydrofolate synthetase (EC 6.3.4.3), was synthesized from formyl fluoride and inorganic phosphateExpand
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