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Production of the antimalarial drug precursor artemisinic acid in engineered yeast
The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid. Expand
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids. Expand
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass. Expand
High-level semi-synthetic production of the potent antimalarial artemisinin
The strains and processes described here form the basis of a viable industrial process for the production of semi-synthetic artemisinin to stabilize the supply of art Artemisinin for derivatization into active pharmaceutical ingredients (for example, artesunate) for incorporation into ACTs. Expand
Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids
This DSRS substantially improved the stability of biodiesel-producing strains and increased the titer and yield threefold and can be extended to many other biosynthetic pathways to balance metabolism, thereby increasing product titers and conversion yields and stabilizing production hosts. Expand
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
Saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms were substituted for Clostridial enzymes and their effect on n- butanol production was compared. Expand
Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals.
This work engineered the budding yeast Saccharomyces cerevisiae to produce fatty acid-derived biofuels and chemicals from simple sugars and envision that this approach will provide a scalable, controllable and economic route to this important class of chemicals. Expand
Synthetic protein scaffolds provide modular control over metabolic flux
Engineered metabolic pathways constructed from enzymes heterologous to the production host often suffer from flux imbalances, as they typically lack the regulatory mechanisms characteristic ofExpand
Retrograde Signaling by the Plastidial Metabolite MEcPP Regulates Expression of Nuclear Stress-Response Genes
It is proposed that the MEP pathway, in addition to producing isoprenoids, functions as a stress sensor and a coordinator of expression of targeted stress-responsive nuclear genes via modulation of the levels of MEcPP, a specific and critical retrograde-signaling metabolite. Expand
Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin
Progress is described toward the goal of developing a supply of semisynthetic artemisinin based on production of the art Artemisinin precursor amorpha-4,11-diene by fermentation from engineered Saccharomyces cerevisiae, and its chemical conversion to dihydroartemisinic acid, which can be subsequently converted to artemis inin. Expand