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Characterization of individual polynucleotide molecules using a membrane channel.
We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only aExpand
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Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules.
Single molecules of DNA or RNA can be detected as they are driven through an alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide mustExpand
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Molecular-scale structural and functional characterization of sparsely tethered bilayer lipid membranes
Surface-tethered biomimetic bilayer membranes (tethered bilayer lipid membranes (tBLMs)) were formed on gold surfaces from phospholipids and a synthetic 1-thiahexa(ethylene oxide) lipid, WC14. TheyExpand
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Binding of neomycin to phosphatidylinositol 4,5-bisphosphate (PIP2).
Schacht (Schacht, J. (1976) J. Neurochem. 27, 1119-1124) demonstrated that neomycin, an aminoglycoside antibiotic, binds with high affinity to phosphatidylinositol 4,5-bisphosphate (PIP2). WeExpand
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Driven DNA transport into an asymmetric nanometer-scale pore.
To understand the mechanism by which individual DNA molecules enter nanometer-scale pores, we studied the concentration and voltage dependence of polynucleotide-induced ionic-current blockades of aExpand
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The kinetic mechanism by which CCCP (carbonyl cyanidem-Chlorophenylhydrazone) transports protons across membranes
SummaryWe demonstrate that a simple kinetic model describes the transport of protons across lipid bilayer membranes by the weak acid CCCP (carbonyl cyanidem-chlorophenylhydrazone). Four parametersExpand
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Dynamics and Free Energy of Polymers Partitioning into a Nanoscale Pore
Membrane-bound proteinaceous nanoscale pores allow us to simultaneously observe the thermodynamic and kinetic properties of differently sized polymers within their confines. We determine the dynamicExpand
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Protonation dynamics of the alpha-toxin ion channel from spectral analysis of pH-dependent current fluctuations.
To probe protonation dynamics inside the fully open alpha-toxin ion channel, we measured the pH-dependent fluctuations in its current. In the presence of 1 M NaCl dissolved in H2O and positiveExpand
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On 'three decades of nanopore sequencing'
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Single-molecule mass spectrometry in solution using a solitary nanopore
We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) moleculesExpand
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