• Publications
  • Influence
Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633
An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity and possessed a high substrate specificity for dipeptides and alpha-H amino acid amides. Expand
New developments in the chemo-enzymatic production of amino acids.
Chemo-enzymatic methods for the synthesis of enantiomerically pure alpha-alkyl-alpha-amino acids are reviewed and a goal-orientated approach towards the selective generation of these novel enzyme activities using in vivo protein engineering techniques is highlighted. Expand
Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase
SummaryFavourable reaction conditions for the enzymatic production of 1-kestose by sucrose-1F-fructosyltransferase, SFT (EC from Aspergillus phoenicis CBS 294.80 mycelium were established.Expand
Synthesis of optically pure .alpha.-alkylated .alpha.-amino acids and a single-step method for enantiomeric excess determination
Determination de l'exces enantiomere par reaction d'α-alkyl α-aminoacides avec le chlorure de l'acide chloro-2 propionique
Peptide Helices as Rigid Molecular Rulers: A Conformational Study of Isotactic Homopeptides from α-Methyl-α-isopropylglycine, [L-(αMe)Val]n†
Terminally blocked, isotactic homopeptides from the sterically demanding α-methylvaline of general formula Y-[L-(αMe)Val]n-OtBu (Y = Z, pBrBz, Ac; n = 2–8) have been prepared step-by-step in solutionExpand
Enzymatic resolution of 1‐phenyl‐1,2‐ethanediol by enantioselective oxidation: Overcoming product inhibition by continuous extraction
The reaction investigated is the kinetic resolution of racemic 1‐phenyl‐1,2‐ethanediol by glycerol dehydrogenase (GDH), which causes a strong product inhibition and reacts in a chemical reaction with the cofactor lowering its active concentration. Expand
Nitroxyl peptides as catalysts of enantioselective oxidations.
These findings are rationalized by proposing models for the diastereomeric intermediates between (R)-[and (S)]-1-phenylethanol and the catalyst Fmoc-TOAC-L-(alphaMe)Val-NHiPr, based on the X-ray diffraction structure of the latter. Expand
Mag: a Cα-Methylated, Side-chain Unsaturated α-Amino Acid. Introduction into Model Peptides and Conformational Preference
Abstract By a chemo-enzymatic approach we synthesized the chiral, Cα-methylated α-amino acid Mag, characterized by a side-chain CγCδ bond. We also prepared a series of model peptides containing MagExpand
Helix induction potential of N-terminal α-methyl, α-amino acids
A series of longer analogues of the C-peptide of RNAse A has been synthesized with the aim of assessing the helix induction potential in water of α-methyl, α-amino acids at the N-terminus of theExpand