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Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633
TLDR
An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity and possessed a high substrate specificity for dipeptides and alpha-H amino acid amides. Expand
New developments in the chemo-enzymatic production of amino acids.
TLDR
Chemo-enzymatic methods for the synthesis of enantiomerically pure alpha-alkyl-alpha-amino acids are reviewed and a goal-orientated approach towards the selective generation of these novel enzyme activities using in vivo protein engineering techniques is highlighted. Expand
Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase
SummaryFavourable reaction conditions for the enzymatic production of 1-kestose by sucrose-1F-fructosyltransferase, SFT (EC 2.4.1.99) from Aspergillus phoenicis CBS 294.80 mycelium were established.Expand
Synthesis of optically pure .alpha.-alkylated .alpha.-amino acids and a single-step method for enantiomeric excess determination
Determination de l'exces enantiomere par reaction d'α-alkyl α-aminoacides avec le chlorure de l'acide chloro-2 propionique
Peptide Helices as Rigid Molecular Rulers: A Conformational Study of Isotactic Homopeptides from α-Methyl-α-isopropylglycine, [L-(αMe)Val]n†
Terminally blocked, isotactic homopeptides from the sterically demanding α-methylvaline of general formula Y-[L-(αMe)Val]n-OtBu (Y = Z, pBrBz, Ac; n = 2–8) have been prepared step-by-step in solutionExpand
Enzymatic resolution of 1‐phenyl‐1,2‐ethanediol by enantioselective oxidation: Overcoming product inhibition by continuous extraction
TLDR
The reaction investigated is the kinetic resolution of racemic 1‐phenyl‐1,2‐ethanediol by glycerol dehydrogenase (GDH), which causes a strong product inhibition and reacts in a chemical reaction with the cofactor lowering its active concentration. Expand
Nitroxyl peptides as catalysts of enantioselective oxidations.
TLDR
These findings are rationalized by proposing models for the diastereomeric intermediates between (R)-[and (S)]-1-phenylethanol and the catalyst Fmoc-TOAC-L-(alphaMe)Val-NHiPr, based on the X-ray diffraction structure of the latter. Expand
Mag: a Cα-Methylated, Side-chain Unsaturated α-Amino Acid. Introduction into Model Peptides and Conformational Preference
Abstract By a chemo-enzymatic approach we synthesized the chiral, Cα-methylated α-amino acid Mag, characterized by a side-chain CγCδ bond. We also prepared a series of model peptides containing MagExpand
Helix induction potential of N-terminal α-methyl, α-amino acids
A series of longer analogues of the C-peptide of RNAse A has been synthesized with the aim of assessing the helix induction potential in water of α-methyl, α-amino acids at the N-terminus of theExpand
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