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Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae.
TLDR
The identification of the 68-kDa glycoprotein as a membrane-bound form of alkaline phosphatase the authors term HvALP as a Cry1Ac binding protein that is present at reduced levels in brush border membrane vesicles from YHD2 larvae is proposed. Expand
Binding Analyses of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of Ostrinia nubilalis
TLDR
It is speculated that isoforms of aminopeptidase and cadherin in the brush border membrane serve as Cry1Ab, Cry1Ac, and Cry1F binding proteins. Expand
Identification of novel Cry1Ac binding proteins in midgut membranes from Heliothis virescens using proteomic analyses.
TLDR
Proteins such as aminopeptidases and alkaline phosphatases, both glycosyl-phosphatidyl-inositol (GPI) anchored proteins, were previously identified as Cry1Ac binding proteins in the Heliothis virescens midgut, but additional toxin binding proteins were predicted based on MS/MS fragmentation and de novo sequencing. Expand
Synergism of Bacillus thuringiensis toxins by a fragment of a toxin-binding cadherin
TLDR
It is reported that a peptide containing this region expressed in Escherichia coli functions as a synergist of Cry1A toxicity against lepidopteran larvae and has important implications related to the use of this peptide to enhance insecticidal activity of Bt toxin-based biopesticides and transgenic Bt crops. Expand
Mechanism and DNA-based detection of field-evolved resistance to transgenic Bt corn in fall armyworm (Spodoptera frugiperda)
TLDR
It is shown that field-evolved resistance to Cry1Fa Bt corn in Puerto Rico is closely linked to a mutation in an ATP Binding Cassette subfamily C2 (ABCC2) gene that functions as a Cry1 Fa receptor in susceptible insects. Expand
A Novel Tenebrio molitor Cadherin Is a Functional Receptor for Bacillus thuringiensis Cry3Aa Toxin*
TLDR
The functional role of TmCad1 as a Cry3Aa receptor in T. molitor larvae is demonstrated and similarities between the mode of action of Cry toxins in Lepidoptera and Coleoptera are revealed. Expand
Comparative molecular analyses of invasive fall armyworm in Togo reveal strong similarities to populations from the eastern United States and the Greater Antilles
TLDR
Genetic methods were used to characterize noctuid specimens infesting multiple cornfields in the African nation of Togo that were tentatively identified as fall armyworms by morphological criteria, demonstrating the usefulness of genetic surveys to characterize fall armyworm populations from Africa. Expand
Methods for discovery and characterization of cellulolytic enzymes from insects
TLDR
The diverse methodologies used to detect, quantify, purify, clone and express cellulolytic enzymes from insects, as well as their advantages and limitations are described. Expand
Prospecting for cellulolytic activity in insect digestive fluids.
TLDR
The data suggests that a phylogenetic relationship may exist for the origin of cellulolytic enzymes in insects, and that cellulase activity levels correlate with taxonomic classification, probably reflecting differences in plant host or feeding strategies. Expand
MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth
TLDR
A crucial role for ALP and ABCC genes in field-evolved resistance to Cry1Ac is highlighted and a novel trans-regulatory signaling mechanism responsible for modulating the expression of these pivotal genes in P. xylostella is revealed. Expand
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