• Publications
  • Influence
Efficient genome editing in zebrafish using a CRISPR-Cas system.
TLDR
It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
CRISPR-Cas systems for editing, regulating and targeting genomes
TLDR
A modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells, which will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.
High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
TLDR
It is found that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface, and off-target cleavage of CRISPR-associated (Cas)9-based RGNs is characterized.
GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
TLDR
The experiments define the most rigorous framework for genome-wide identification of RGN off-target effects to date and provide a method for evaluating the safety of these nucleases before clinical use.
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
TLDR
It is shown that the commonly used Streptococcus pyogenes Cas9 (SpCas9) can be modified to recognize alternative PAM sequences using structural information, bacterial selection-based directed evolution, and combinatorial design, and the feasibility of engineering a wide range of Cas9s with altered and improved PAM specificities is established.
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
TLDR
It is reported that truncated gRNAs, with shorter regions of target complementarity <20 nucleotides in length, can decrease undesired mutagenesis at some off-target sites by 5,000-fold or more without sacrificing on-target genome editing efficiencies.
TALENs: a widely applicable technology for targeted genome editing
TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.
CRISPR RNA-guided activation of endogenous human genes
TLDR
This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
...
...