Glycine potentiates the NMDA response in cultured mouse brain neurons
- J. Johnson, P. Ascher
- BiologyNature
- 5 February 1987
G glycine may facilitate excitatory transmission in the brain through an allosteric activation of the NMDA receptor, and can be observed in outside-out patches as an increase in the frequency of opening of the channels activated by NMDA agonists.
Trapping channel block of NMDA-activated responses by amantadine and memantine.
- T. Blanpied, F. Boeckman, E. Aizenman, J. Johnson
- BiologyJournal of Neurophysiology
- 1997
We investigated the mechanisms by which the antiparkinsonian and neuroprotective agents amantadine and memantine inhibit responses to N-methyl-D-aspartic acid (NMDA). Whole cell recordings were…
Permeant ion regulation of N-methyl-D-aspartate receptor channel block by Mg(2+).
- S. Antonov, J. Johnson
- BiologyProceedings of the National Academy of Sciences…
- 7 December 1999
The model provides an explanation for the strength of the voltage dependence of Mg(o)(2+) block and quantifies the interdependence of permanent and blocking ion binding to NMDA receptors.
Voltage-dependent block by intracellular Mg2+ of N-methyl-D-aspartate-activated channels.
- J. Johnson, P. Ascher
- BiologyBiophysical Journal
- 1 May 1990
Voltage‐dependent interaction of open‐channel blocking molecules with gating of NMDA receptors in rat cortical neurons.
- S. Antonov, J. Johnson
- BiologyJournal of Physiology
- 1 June 1996
The data suggest that there are two blocking sites at different depths within the NMDA‐activated channel, and that channel closure is prevented when any of the IEM drugs occupy the shallow blocking site.
Glycine-insensitive desensitization of NMDA responses in cultured mouse embryonic neurons
- W. Sather, J. Johnson, G. Henderson, P. Ascher
- BiologyNeuron
- 1 May 1990
Equilibrium and kinetic study of glycine action on the N‐methyl‐D‐aspartate receptor in cultured mouse brain neurons.
- J. Johnson, P. Ascher
- BiologyJournal of Physiology
- 1 September 1992
1. The characteristics of the activation of the N‐methyl‐D‐aspartate (NMDA) response by glycine were studied using whole‐cell and outside‐out patch clamp recording techniques. 2. Glycine…
Competitive antagonists and partial agonists at the glycine modulatory site of the mouse N‐methyl‐D‐aspartate receptor.
- G. Henderson, J. Johnson, P. Ascher
- BiologyJournal of Physiology
- 1 November 1990
Results can be interpreted by assuming that Kyn and 7‐Cl‐Kyn are competitive antagonists of glycine, HA‐966 and D‐cycloserine are partial agonists, in the absence of added glycine some glycine is present in the extracellular solution and the response in the total absence of glycines is very small or negligible.
Mechanisms of glutamate‐stimulated Mg2+ influx and subsequent Mg2+ efflux in rat forebrain neurones in culture.
- A. Stout, Y. Li-Smerin, J. Johnson, I. Reynolds
- BiologyJournal of Physiology
- 1 May 1996
The results suggest that glutamate‐stimulated increases in [Mg2+]i occur in the cultured forebrain neurones from fetal rats in the absence of extracellular Na+ (Nao+) and Ca2+ (Cao2+), and that NMDA channel‐permeant ions N‐methyl‐D‐glucamine (NMDG), Tris or sucrose did not inhibit recovery significantly more than either manipulation alone.
Kinetics of the block by intracellular Mg2+ of the NMDA‐activated channel in cultured rat neurons.
- Y. Li-Smerin, J. Johnson
- BiologyJournal of Physiology
- 15 February 1996
The very fast rate constants of the block by Mgi2+ explain why channel flicker was not fully resolvable during block of the NMDA‐activated single‐channel current, and an energy profile of three barriers and two binding sites for Mg2+ is proposed.
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