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On Product MV-Algebras
In this paper we apply the notion of the product MV-algebra in accordance with the definition given by B. Riečan. We investigate the convex embeddability of an MV-algebra into a product MV-algebra.Expand
Positive cooperativity of acetylcholine and other agonists with allosteric ligands on muscarinic acetylcholine receptors.
The discovery that it is possible to increase the affinity of muscarinic receptors for their agonists by allosteric modulators offers a new way to subtype-specific pharmacological enhancement of transmission at cholinergic (muscarinic) synapses. Expand
Constitutive activity of the M1–M4 subtypes of muscarinic receptors in transfected CHO cells and of muscarinic receptors in the heart cells revealed by negative antagonists
It is concluded that the M1–M4 subtypes of muscarinic receptors are constitutively active in the CHO cell lines expressing them and in cardiomyocytes and that atropine and NMS act as negative antagonists on these receptor subtypes by stabilizing them in the inactive conformation. Expand
Evidence for a Tandem Two-site Model of Ligand Binding to Muscarinic Acetylcholine Receptors*
Experimental data on [ 3H]NMS and [3H]QNB association and dissociation perfectly agree with the predictions of the tandem two-site model, which support a model in which the receptor binding domain has two tandemly arranged subsites for classical ligands, a peripheral one and a central one. Expand
Multiplication and distribution of Aujeszky's disease (pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection
Though multiplication and dissemination of ADV occurred, vaccinated pigs did not show clinical symptoms of Aujeszky's disease and the role of haematogenic or lymphatic dissemination of the virus by WBC and to humoral and cell-mediated immunity in vaccinated pigs is discussed. Expand
Role of Receptor Protein and Membrane Lipids in Xanomeline Wash-Resistant Binding to Muscarinic M1 Receptors
It is suggested that the binding of xanomeline involves interhelical penetration of M1 muscarinic receptor by xanumeline's O-alkyl chain and interaction with membrane lipids surrounding the receptor. Expand
Use of a Sandwich Enzyme-linked Immunosorbent Assay Strategy to Study Mechanisms of G Protein-coupled Receptor Assembly*
Interestingly, incubation with classical muscarinic agonists and antagonists or allosteric ligands led to significant increases in the efficiency of fragment association (particularly upon substitution of the conserved proline residues), indicating that all of these ligands can act as “anchors” between the m3-trunk and m3 -tail fragments. Expand
Activation of muscarinic acetylcholine receptors via their allosteric binding sites.
It is found that the muscarinic receptor allosteric modulators alcuronium, gallamine, and strychnine alter the synthesis of cAMP in Chinese hamster ovary cells expressing the M2 or the M4 subtype ofMuscarinic receptors in the same direction as the agonist carbachol. Expand
Allosteric modulation by persistent binding of xanomeline of the interaction of competitive ligands with the M1 muscarinic acetylcholine receptor.
The pharmacological characteristics of this unique mode of xanomeline binding and the effects of this binding on the interaction of classical competitive ligands with the receptor in CHO cells that express the M(1) muscarinic receptor are investigated. Expand
Protection by Alcuronium of Muscarinic Receptors Against Chemical Inactivation and Location of the Allosteric Binding Site for Alcuronium
The effect of alcuronium indicates that the drug interferes with the access of chemical modifiers to the muscarinic sites, and appears to be best explained on the assumption that theDrug binds in close vicinity of the “classical” musCarinic site and sterically blocks the access to this site. Expand