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Embryonic stem cell lines derived from human blastocysts.
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primateExpand
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes.
The human ES cell--derived cardiomyocytes displayed structural and functional properties of early-stage cardiomers, which may have significant impact on the study of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering. Expand
Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture.
The clonal derivation of two human ES cell lines, H9.1 and H.2, demonstrates the pluripotency of single human ES cells, the maintenance of pluripOTency during an extended period of culture, and the long-term self-renewing properties of cultured human ES Cells. Expand
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation andExpand
Differences between human and mouse embryonic stem cells.
The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions. Expand
Characterization of the expression of MHC proteins in human embryonic stem cells
The results demonstrate that human ES cells can express high levels of MHC-I proteins and thus may be rejected on transplantation, and isotyped several of the published ES cell lines for their human leukocyte antigens. Expand
Electromechanical integration of cardiomyocytes derived from human embryonic stem cells
The potential of hES-cell cardiomyocytes to act as a rate-responsive biological pacemaker and for future myocardial regeneration strategies are demonstrated. Expand
Endothelial cells derived from human embryonic stem cells
The differentiation steps of human embryonic stem cells into endothelial cells forming vascular-like structures are described and it is shown that the isolated embryonic PECAM1+ cells, grown in culture, display characteristics similar to vessel endothelium. Expand
Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.
This analysis sets the stage for directing differentiation of human ES cells in culture and indicates that multiple human cell types may be enriched in vitro by specific factors. Expand
Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells1
A novel feeder layer-free culture system for hES cells is presented, based on medium supplemented with 15% serum replacement, a combination of growth factors including transforming growth factor β1 (TGFβ1), leukemia inhibitory factor, basic fibroblast growth factor, and fibronectin matrix, which maintains all ES cell features after prolonged culture. Expand