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Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors
Recent optimized procedures for CGH are described, including DNA labeling, hybridization, fluorescence microscopy, digital image analysis, data interpretation, and quality control, emphasizing those steps that are most critical.
In vivo amplification of the androgen receptor gene and progression of human prostate cancer
This work has identified a similar molecular mechanism in vivo for endocrine treatment failure in human prostate cancer which involves amplification of the androgen receptor (AR) gene.
Prognostic significance of elevated cyclooxygenase-2 expression in breast cancer.
The results indicate that elevated Cox-2 expression is more common in breast cancers with poor prognostic characteristics and is associated with an unfavorable outcome, and support efforts to initiate clinical trials on the efficacy of Cox- 2 inhibitors in adjuvant treatment of breast cancer.
Poor prognosis in carcinoma is associated with a gene expression signature of aberrant PTEN tumor suppressor pathway activity
It is indicated that aberrant PI3K pathway signaling is strongly associated with metastasis and poor survival across carcinoma types, highlighting the enormous potential impact on patient survival that pathway inhibition could achieve.
Adjuvant docetaxel or vinorelbine with or without trastuzumab for breast cancer.
A short course of trastuzumab administered concomitantly with docetaxel or vinorelbine is effective in women with breast cancer who have an amplified HER2/neu gene.
Amplification of HER-2 in gastric carcinoma: association with Topoisomerase IIalpha gene amplification, intestinal type, poor prognosis and sensitivity to trastuzumab.
HER-2/neu amplification is common in the intestinal type of gastric carcinoma, and it is associated with a poor outcome, and this hypothesis deserves to be investigated in clinical trials.
Genetic changes in primary and recurrent prostate cancer by comparative genomic hybridization.
The CGH results indicate that losses of several chromosomal regions are common genetic changes in primary tumors, suggesting that deletional inactivation of putative tumor suppressor genes in these chromosomal sites is likely to underlie development of prostate cancer.
ImmunoRatio: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67
- Vilppu J. Tuominen, Sanna Ruotoistenmäki, A. Viitanen, M. Jumppanen, J. Isola
- BiologyBreast Cancer Research
- 27 July 2010
It is anticipated that free web applications, such as ImmunoRatio, will make the quantitative image analysis of ER, PR, and Ki-67 easy and straightforward in the diagnostic assessment of breast cancer specimens.
Chromogenic in situ hybridization: a practical alternative for fluorescence in situ hybridization to detect HER-2/neu oncogene amplification in archival breast cancer samples.
Androgen receptor gene amplification: a possible molecular mechanism for androgen deprivation therapy failure in prostate cancer.
Failure of conventional androgen deprivation therapy in prostate cancer may be caused by a clonal expansion of tumor cells that are able to continue androgen-dependent growth despite of the low concentrations of serum androgens.