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Estimation and Diversity of Phylloplane Mycobiota on Selected Plants in a Mediterranean–Type Ecosystem in Portugal
TLDR
Interestingly, a few species seemed to be associated with a particular plant, notably in the case of the evergreen shrub C. albidus, suggesting that such species might be genuine phylloplane inhabitants (or at least of aerial plant surfaces) even though they appeared not to display host specificity.
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In Situ Accessibility of Small-Subunit rRNA of Members of the Domains Bacteria, Archaea, and Eucarya to Cy3-Labeled Oligonucleotide Probes
TLDR
A consensus model for the accessibility of the small-subunit rRNA to oligonucleotide probes is proposed which uses 60 homolog target sites of the three prokaryotic 16S rRNA molecules.
View on ASMUSA
Phylloplane yeasts from Portugal: seven novel anamorphic species in the Tremellales lineage of the Hymenomycetes (Basidiomycota) producing orange-coloured colonies.
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Application of fluorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations.
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In Situ Accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-Labeled Oligonucleotide Probes Comprising the D1 and D2 Domains
TLDR
Flow cytometry is used to determine whole-cell fluorescence intensities with a set of 32 Cy3-labeled oligonucleotide probes covering the full length of the D1 and D2 domains in the 26S rRNA of Saccharomyces cerevisiae PYCC 4455T, contributing to a more rational design of species-specific probes for yeast identification and monitoring.
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A low cost, safe, disposable, rapid and self-sustainable paper-based platform for diagnostic testing: lab-on-paper
TLDR
The obtained results with the above-mentioned proof-of-concept sensors are thus promising towards the future development of simple and cost-effective paper-based diagnostic devices.
View on IOP Publishing
Gold on paper-paper platform for Au-nanoprobe TB detection.
TLDR
A paper-based platform capable of integrating a previously developed Au-nanoprobe based MTBC detection assay is reported, called "Gold on Paper", allowing unequivocal identification of MTBC members and can be performed without specialised laboratory equipment.
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Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin.
TLDR
The results confirmed the presence of both Cryptococcus species in the Mediterranean environment, and showed that both carob and olive trees represent an important niche for these yeasts.
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Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains.
TLDR
The experimental proof of concept of wax-printed μPADs functionalized with CBM-ZZ for DNA detection at room temperature presented in this study constitutes an important step toward the development of easy to use and affordable molecular diagnostic tests.
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Ethanol tolerance of sugar transport, and the rectification of stuck wine fermentations.
TLDR
The results challenge the current belief that ethanol tolerance, expressed in terms of cell viability, is a reliable criterion for the selection of yeast strains to restart stuck fermentations, and suggest that this capacity seems to be based on sugar transport and its resistance to ethanol.
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