• Publications
  • Influence
Membrane budding and scission by the ESCRT machinery: it's all in the neck
TLDR
The endosomal sorting complexes required for transport (ESCRTs) catalyse one of the most unusual membrane remodelling events in cell biology, which is crucial for many processes, including the biogenesis of multivesicular bodies, viral budding, cytokinesis and, probably, autophagy.
The ESCRT complexes: structure and mechanism of a membrane-trafficking network.
  • J. Hurley, S. Emr
  • Biology
    Annual review of biophysics and biomolecular…
  • 11 May 2006
The ESCRT complexes and associated proteins comprise a major pathway for the lysosomal degradation of transmembrane proteins and are critical for receptor downregulation, budding of the HIV virus,
Molecular Mechanism of Multivesicular Body Biogenesis by ESCRT Complexes
TLDR
It is shown that ESCRT-0 forms domains of clustered cargo but does not deform membranes, which explains how the ESCRTs direct membrane budding and scission from the cytoplasmic side of the bud without being consumed in the reaction.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
ESCRT complexes and the biogenesis of multivesicular bodies.
  • J. Hurley
  • Biology
    Current opinion in cell biology
  • 1 February 2008
Membrane scission by the ESCRT-III complex
TLDR
Three subunits of ESCRT-III, Vps20, Snf7 and Vps24, are sufficient to detach intralumenal vesicles and are reconstituted and visualized by fluorescence microscopy.
Structural mechanism for sterol sensing and transport by OSBP-related proteins
TLDR
A model is proposed in which sterol and membrane binding promote reciprocal conformational changes that facilitate a sterol transfer and signalling cycle and shows that sterol binding closes the lid and stabilizes a conformation favouring transport across aqueous barriers and signal transmission.
Structure and lipid transport mechanism of a StAR-related domain
TLDR
Based on the START domain structure and cholesterol binding stoichiometry, it is proposed that StAR acts by shuttling cholesterol molecules one at a time through the intermembrane space of the mitochondrion.
The ESCRT complexes
  • J. Hurley
  • Biology
    Critical reviews in biochemistry and molecular…
  • 18 November 2010
TLDR
This review will describe the structure and function of the six complexes noted above and summarize current knowledge of their mechanistic roles in cellular pathways and in disease.
Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission
TLDR
Data reveal that the arrival of ESCRT-III and VPS4 correlates both spatially and temporally with the abscission event suggests a direct role for these proteins in cytokinetic membrane abscissions.
...
...