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Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination…
Arterial-Venous Segregation by Selective Cell Sprouting: An Alternative Mode of Blood Vessel Formation
A third mode of blood vessel formation is uncovered in zebrafish whereby the first embryonic artery and vein, two unconnected blood vessels, arise from a common precursor vessel, a process essential for vascular development.
A dual role for ErbB2 signaling in cardiac trabeculation
It is proposed that cardiac trabeculation is initiated by directional cardiomyocyte migration rather than oriented cell division, and that ErbB2 cell-autonomously regulates this process.
Genetic and Physiologic Dissection of the Vertebrate Cardiac Conduction System
This work identified and analyzed four distinct stages of cardiac conduction development that correspond to cellular and anatomical changes of the developing heart and observed that epigenetic factors, such as hemodynamic flow and contraction, regulate the fast conduction network of this specialized electrical system.
Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM).
Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in…
High-speed imaging of developing heart valves reveals interplay of morphogenesis and function
High-speed fluorescence microscopy is used to investigate AVV morphogenesis in zebrafish at cellular resolution and finds that valve leaflets form directly through a process of invagination, rather than first forming endocardial cushions.
Zebrafish model for human long QT syndrome
It is shown that complete loss of functional IKr in embryonic hearts leads to ventricular cell membrane depolarization, inability to generate action potentials (APs), and disrupted calcium release, and a small hyperpolarizing current restores spontaneous APs.
Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope
This study systematically quantified the viability and mobility of zebrafish embryos mounted under more suitable conditions and found that tubes made of fluorinated ethylene propylene filled with low concentrations of agarose or methylcellulose provided an optimal balance between sufficient confinement of the living embryo in a physiological environment over 3 days and optical clarity suitable for fluorescence imaging.
A guide to light-sheet fluorescence microscopy for multiscale imaging
This work elucidate the key developments and define a simple set of underlying principles governing LSFM, which aim to clarify the decisions to be made for those who wish to develop and use bespoke light-sheet systems and to assist in identifying the best approaches to apply this powerful technique to myriad biological questions.