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Competition between chromatin and transcription complex assembly regulates gene expression during early development
Xenopus early development is characterized by a generalized absence of transcription, which resumes at the midblastula transition (MBT). We analyzed this regulation using a plasmid containing theExpand
2 Yeast RNA Polymerase Subunits and Genes
OVERVIEW Yeast RNA polymerases A(I), B(II), and C(III) are organized around a common core of subunits related to the bacterial core enzyme (β′ βα 2 ) and share a set of five small subunits (ABC27,Expand
Publisher Summary This chapter describes the eukaryotic RNA polymerases. The RNA polymerase A was subjected to mild urea treatment. The enzyme was treated with 4M urea that resulted in 80%Expand
Dissociation of two polypeptide chains from yeast RNA polymerase A.
Yeast RNA polymerase A (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase; EC can be converted to a new form of enzyme, called RNA polymerase A*, which is lackingExpand
Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C.
Fractionation of transcription extracts has led to the identification of multiple transcription factors specific for each form of nuclear RNA polymerase. Accurate transcription in vitro of the yeastExpand
RNA polymerase III and class III transcription factors from Saccharomyces cerevisiae.
Publisher Summary RNA polymerase III is specifically recruited at the transcription start sites via a cascade of protein-protein-DNA interactions involving various transcription factors. Remarkably,Expand
The TATA-binding protein participates in TFIIIB assembly on tRNA genes.
The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we showExpand
A yeast activity can substitute for the HeLa cell TATA box factor
Most class B (II) promoter regions from higher eukaryotes contain the TATA box and upstream and enhancer elements1. Both the upstream and enhancer elements and their cognate factors have regulatoryExpand
Archaebacteria and eukaryotes possess DNA‐dependent RNA polymerases of a common type.
DNA‐dependent RNA polymerases of archaebacteria not only resemble the nuclear RNA polymerases of eukaryotes rather than the eubacterial enzymes in their complex component patterns but also showExpand
A general upstream binding factor for genes of the yeast translational apparatus.
Fractionation of yeast extracts on heparin‐agarose revealed the presence of a DNA footprinting activity that interacted specifically with the 5′‐upstream region of TEF1 and TEF2 genes coding for theExpand