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Use of a Tissue Blotting Immunoassay to Examine the Distribution of Pineapple Closterovirus in Hawaii.
Specific monoclonal antibodies made to a pineapple closterovirus (PCV) were used in a tissue blotting immunoassay (TBIA) for the detection of PCV in pineapple, suggesting that PCV may be involved in MWP. Expand
Diversity and Mealybug Transmissibility of Ampeloviruses in Pineapple.
Plants infected with PMWaV-3 that were continuously exposed to mealybugs did not develop symptoms of MWP in the absence of PMWaVs, and the potential deletion mutant spanning the N-terminal of the HSP70 region was obtained from a pineapple accession from Zaire maintained at the USDA-ARS National Clonal Germplasm Repository in Hawaii. Expand
Transmission of Pineapple Mealybug Wilt-Associated Virus by Two Species of Mealybug (Dysmicoccus spp.).
All stages of D. neobrevipes acquired PMWaV, although vector efficiency decreased significantly in older adult females, and the presence of ants was correlated with an increased rate of virus spread when caged with D. brevipes. Expand
Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1
The complete genome of PMWaV-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family and unusual for an ampelovirus. Expand
Transmission, movement and inactivation of cymbidium mosaic and odontoglossum ringspot viruses.
Transmission and movement studies were conducted on orchids mechanically inoculated with cymbidium mosaic virus (CyMV) or odontoglossum ringspot virus (ORSV). Transmission of CyMV and ORSV toExpand
Closterovirus infection and mealybug exposure are necessary for the development of mealybug wilt of pineapple disease.
A PMWaV-2-specific monoclonal antibody was produced that decorated the particles in immunosorbent electron microscopy and detected the virus in tissue blot immunoassays and developed MWP symptoms while sustaining mealybug populations. Expand
The coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants
A lambda ZAP II cDNA library constructed by cloning cDNA prepared from a high molecular weight double-stranded RNA isolated from grapevine leafroll associated closterovirus-3 infected tissues revealed that the consensus amino acid residues of filamentous plant viruses are preserved in the expected locations. Expand
Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2.
The genome of pineapple mealybug wilt-associated closterovirus-2 was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined, andylogenetic analysis revealed that within the family CLOSTEROVIRIDAE: the mealy bug-transmitted PMWaV-2 is more closely related to other mealy bugs than to those which are transmitted by aphids or whiteflies. Expand
Use of monoclonal antibodies to characterize grapevine leafroll associated closteroviruses.
Sensitivity of the monoclonal antibodies were very good for the detection of the virus in grape leaf tissue in double antibody sandwich ELISA and dot-immunoblotting assays. Expand
Yield Impact and Spread of Pineapple mealybug wilt associated virus-2 and Mealybug Wilt of Pineapple in Hawaii.
Yield reductions were dependent on time of MWP symptom development; the earlier the expression of symptoms the greater the impact on fruit yields, and patterns of both PMWaV-2 incidence and MWP were overdispersed. Expand