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Comparative Analysis of Regulatory Elements between Escherichia coli and Klebsiella pneumoniae by Genome-Wide Transcription Start Site Profiling
TLDR
A new dimension of comparative genomics such that a comparison of two genomes needs to be comprehensive over all levels of genome organization is revealed.
An Experimentally Validated Genome-Scale Metabolic Reconstruction of Klebsiella pneumoniae MGH 78578, iYL1228
TLDR
The genome-scale metabolic reconstruction for K. pneumoniae presented here provides an experimentally validated in silico platform for further studies of this important industrial and biomedical organism.
Multiple-omic data analysis of Klebsiella pneumoniae MGH 78578 reveals its transcriptional architecture and regulatory features
TLDR
A systems biology approach is taken to annotate the K. pneumoniae MGH 78578 genome at the structural and operational levels to provide new insight into fundamental genome-level biology for this important human pathogen.
Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set
TLDR
Heterologous expression of different combinations of putative 2-DOS biosynthetic genes revealed that a subset, gtmB-gtmA-gacH, is responsible for the biosynthesis of this core aminocyclitol moiety of gentamicin.
New olivosyl derivatives of methymycin/pikromycin from an engineered strain of Streptomyces venezuelae.
TLDR
A successful attempt of engineering the deoxysugar pathway for generation of novel hybrid macrolide antibiotics and the 'sugar-flexible' glycosyltransferase (DesVII) was able to attach the intermediate sugar and the new sugar to both 12- and 14-membered macrolactones thus producing quinovose or olivose glycosymethynolide and narbonolide, respectively.
Epidemiological features and genetic variation of human respiratory syncytial virus (HRSV) infection in Chungnam, Korea
TLDR
Investigation of the epidemiological patterns and genetic variation of Human Respiratory Syncytial Virus isolated from Chungnam, Korea, during 2011-2014 found temporal distribution of the HRSV epidemic showed seasonal prevalence for early winter and mainly founded in children’s less than 3 years of age.
New BODIPY derivatives as OFF-ON fluorescent chemosensor and fluorescent chemodosimeter for Cu2+: cooperative selectivity enhancement toward Cu2+.
New 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives (1 and 2) were synthesized as an "off-on" fluorescent chemosensor and fluorescent chemodosimeter for Cu2+ and Pb2+. Compound 1
Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae
TLDR
A successful attempt for a heterologous expression of Tyl PKS is demonstrated in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS using two compatible low-copy plasmids, each under the control of a pikAI promoter.
Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae
TLDR
Results demonstrate successful generation of structurally diverse hybrid macrolides using a S. venezuelae in vivo system and provide further insight into the substrate flexibility of glycosyltransferases.
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