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The Archaeal Lsm Protein Binds to Small RNAs*
TLDR
The deletion of the lsm gene is viable, resulting in a pleiotropic phenotype, indicating that the haloarchaeal Lsm is involved in many cellular processes, which is in congruence with the number of protein interaction partners.
Asymmetric ATP Hydrolysis Cycle of the Heterodimeric Multidrug ABC Transport Complex TmrAB from Thermus thermophilus*
TLDR
It is demonstrated that slow ATP hydrolysis in the newly generated canonic site (D500E) strictly depends on the formation of a posthydrolysis state in the consensus site, indicating an allosteric coupling of both active sites.
Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.
TLDR
Using laser-induced liquid bead ion desorption mass spectrometry (LILBID-MS), the oligomeric structure of ExbB was determined to be mainly hexameric (ExbB(6), with minor amounts of trimeric, dimeric, and monomeric oligomers, and the final complex including TonB is assembled.
Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology
TLDR
The detergent-free synthesis of membrane protein/nanodisc samples and the analysis by LILBID mass spectrometry provide a versatile platform for the analysis of membrane proteins in a native environment.
Helical jackknives control the gates of the double-pore K+ uptake system KtrAB
TLDR
An inactive ADP-bound structure of KtrAB from Vibrio alginolyticus is presented, determined by cryo-electron microscopy, which uncovers a novel regulatory mechanism for ligand-induced action at a distance, and may advance structure-guided drug development.
Biochemical Analysis of the Complex between the Tetrameric Export Adapter Protein Rec of HERV-K/HML-2 and the Responsive RNA Element RcRE pck30
TLDR
Laser-induced liquid beam ion desorption mass spectrometry revealed that Rec forms stable tetramers, which are further stabilized upon RNA binding, and chemical probing of the secondary structure of the RcRE corroborated the theory that the R cRE forms a complex folded structure with seven stem-loop regions.
Acyl modification and binding of mitochondrial ACP to multiprotein complexes.
ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry.
TLDR
The present study with the novel laser induced liquid bead ion desorption mass spectrometry (LILBID-MS) reports on the determination of the subunit composition of the F(1)F(o)-ATP synthase from Bacillus pseudofirmus OF4, that of both bovine heart and, for the first time, of human heart mitochondrial F( 1)F (o)- ATP synthases.
Multiple targets for suppression of RNA interference by tomato aspermy virus protein 2B.
TLDR
The nucleic acid binding properties of the Cucumovirus RNAi suppressor tomato aspermy virus protein 2B (TAV 2B) are reported, showing that TAV 2B binds double-stranded RNA corresponding to siRNAs and miRNAs, as well as single-Stranded RNA oligonucleotides.
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