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Amino Acid Sequence and Molecular Structure of an Alkaline Amylopullulanase from Bacillus That Hydrolyzes α-1,4 and α-1,6 Linkages in Polysaccharides at Different Active Sites*
- Y. Hatada, K. Igarashi, +6 authors S. Ito
- ChemistryThe Journal of Biological Chemistry
- 27 September 1996
An amylopullulanase from alkalophilic Bacillus sp. KSM-1378 hydrolyzes both α-1,6 linkages in pullulan and α-1,4 linkages in other polysaccharides, with maximum activity in each case at an alkaline…
Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16
- T. Kobayashi, Y. Hakamada, +5 authors S. Ito
- Chemistry, MedicineApplied Microbiology and Biotechnology
- 1 July 1995
Alkaline protease (EC 220.127.116.11) activity, suitable for use in detergents, was detected in the alkaline culture medium of Bacillus sp. KSM-K16, which was originally isolated from soil. The enzyme,…
High-resolution crystal structure of M-protease: phylogeny aided analysis of the high-alkaline adaptation mechanism.
The biased distribution and interactions caused by the substituted residues seem to be responsible for stabilization of the conformation in a high-alkaline condition.
Moraxella Species Are Primarily Responsible for Generating Malodor in Laundry
- H. Kubota, Asako Mitani, +5 authors J. Hitomi
- Biology, MedicineApplied and Environmental Microbiology
- 24 February 2012
It is found that M. osloensis exists at a high frequency in various living environments, particularly in laundry in Japan, and has the potential to generate 4M3H in laundry.
Application of the upstream region of a Bacillus endoglucanase gene to high-level expression of foreign genes in Bacillus subtilis.
- Nobuyuki Sumitomo, K. Ozaki, +4 authors S. Ito
- Medicine, BiologyBioscience, biotechnology, and biochemistry
- 23 November 1995
A 0.4-kb ScaI-HpaI fragment, 199 bp upstream of the structural gene for alkaline endoglucanase, from the alkalophilic Bacillus sp. KSM-64, was found to be essential for the extracellular production…
Stress Tolerance of Methylobacterium Biofilms in Bathrooms
- T. Yano, H. Kubota, Junya Hanai, J. Hitomi, H. Tokuda
- Biology, MedicineMicrobes and environments
- 1 December 2012
Bacterium-sized cells were found to be predominant in 42 pink biofilms in Japan using a scanning electron microscope, and Methylobacterium strains detected from all samples in bathrooms by an isolation method exhibited higher tolerance to desiccation than other isolated species on FRP.
A novel species of alkaliphilic Bacillus that produces an oxidatively stable alkaline serine protease
This novel strain produces a new class of protease, an oxidatively stable serine protease that is suitable for use in bleach-based detergents and is proposed as a novel species of alkaliphilic Bacillus.
Amino acid sequence and stereoselective hydrolytic reaction of an endo-1,4-beta-glucanase from a Bacillus strain.
- J. Hitomi, Y. Hatada, S. Kawaminami, S. Kawai, S. Ito
- Biology, MedicineBioscience, biotechnology, and biochemistry
- 23 December 1997
The molecular cloning and sequencing of the gene for the fourth EG (EG-IV) of the organism and the mechanism of its hydrolytic reaction are reported, demonstrating that the hydrolysis of p-nitrophenyl beta-D-cellotrioside by EG-IV proceeded with inversion of the anomeric configuration.
Amino acid sequence and molecular structure of an alkaline amylopullulanase from Bacillus that hydrolyzes alpha-1,4 and alpha-1,6 linkages in polysaccharides at different active sites.
The molecular cloning and sequencing of the gene for and the structure of this enzyme are reported and it is shown that its dual hydrolytic activities are associated with two independent active sites.
Purification and Characterization of 1,2-α-Mannosidase of Aspergillus oryzae
1,2-alpha-Mannosidase was purified approximately 1,400-fold from an enzyme product of Aspergillus oryzae. The enzyme showed a single band in disc gel electrophoresis and the molecular weight was…