Molecular Anatomy of a Trafficking Organelle
EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTION
During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae.
Caveolin, a protein component of caveolae membrane coats
Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase.
Immunoelectron microscopy of infected macrophages and immunoblotting of isolated phagosomes showed that Mycobacterium vacuoles acquire the lysosomal membrane protein LAMP-1, but not the vesicular proton-adenosine triphosphatase (ATPase) responsible for phagosomal acidification.
Role of Escherichia coli Curli Operons in Directing Amyloid Fiber Formation
Biochemical, biophysical, and imaging analyses revealed that fibers produced by Escherichia coli called curli were amyloid, and curli biogenesis was dependent on the nucleation-precipitation machinery requiring the CsgE and CsgF chaperone-like and nucleator proteins, respectively.
Intracellular Bacterial Biofilm-Like Pods in Urinary Tract Infections
It is discovered that the intracellular bacteria matured into biofilms, creating pod-like bulges on the bladder surface, which explains how bladder infections can persist in the face of robust host defenses.
Induction and evasion of host defenses by type 1-piliated uropathogenic Escherichia coli.
Structure and Conformational Changes in NSF and Its Membrane Receptor Complexes Visualized by Quick-Freeze/Deep-Etch Electron Microscopy
Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation
In spite of their seeming unrelatedness, both K+ depletion and hypertonic treatment cause coated pits to disappear, and both induce abnormal clathrin polymerization into empty microcages, which suggests that in both cases, an abnormal formation of microc cages inhibits endocytosis.
Drosophila Spire is an actin nucleation factor
Drosophila protein Spire represents a third class of actin nucleation factor that nucleates new filaments at a rate similar to that of the formin family of proteins but slower than in the activated Arp2/3 complex, and it remains associated with the slow-growing pointed end of the new filament.