• Publications
  • Influence
Translational control in mammalian cells.
  • J. Hershey
  • Chemistry, Medicine
  • Annual review of biochemistry
  • 1991
2 The Pathway and Mechanism of Initiation of Protein Synthesis
This chapter is concerned with the prokaryotic and eukaryotic pathways of initiation, where most translational controls are found, and focuses on how the initiation factors catalyze the binding of the initiator tRNA and mRNA to the small ribosomal subunit. Expand
The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity
It is demonstrated that the p90 ribosomal protein S6 kinase (RSK) phosphorylates eIF4B on the same residue as the S6K and RSK proteins, which increases the interaction of eif4B with the eukaryotic translation initiation factor 3. Expand
Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases
It is proposed that eIF4B may mediate some of the effects of the S6Ks on translation, and specifically phosphorylation of Ser422, located in an RNA‐binding region required for eif4A helicase‐promoting activity. Expand
Upf1 Phosphorylation Triggers Translational Repression during Nonsense-Mediated mRNA Decay
A critical step during NMD is uncovered that converts a pioneer translation initiation complex to a translationally compromised mRNP, and it is shown that phospho-Upf1 functions after nonsense codon recognition during steps that involve the translation initiation factor eIF3 and mRNA decay factors. Expand
Principles of translational control: an overview.
The pathway of protein synthesis is outlined, with special emphasis on the initiation phase, and areas needing further clarification are identified. Expand
Individual Overexpression of Five Subunits of Human Translation Initiation Factor eIF3 Promotes Malignant Transformation of Immortal Fibroblast Cells*
It is hypothesized that eIF3 contributes to hyperactivation of the translation initiation machinery and thereby may play an important role in neoplasia, suggesting that the initiation factors may be promising therapeutic targets for treating cancer. Expand
Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae.
  • H. Kang, J. Hershey
  • Biology, Medicine
  • The Journal of biological chemistry
  • 11 February 1994
The results suggest that the activity of eIF-5A may not be absolutely essential for general protein synthesis, but may be selectively required for translation of certain mRNAs and/or may be involved in some other aspect of cell metabolism. Expand
Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3
The results show that the 13-subunit complex can be maintained intact in the gas phase, enabling us to establish unambiguously its stoichiometry and its overall subunit architecture via tandem mass spectrometry and solution disruption experiments. Expand