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Delivering CRISPR: a review of the challenges and approaches
TLDR
The focus then turns to the most difficult barrier to potential in vivo use of CRISPR/Cas9, delivery, and detail the various cargos and delivery vehicles reported for CRISpr/ Cas9, including physical delivery vehicles, viral delivery methods, and non-viral delivery methods.
Supramolecular self-assembled chaos: polyphenolic lignin's barrier to cost-effective lignocellulosic biofuels.
TLDR
Supramolecular self-assembly, nano-mechanical properties of lignin-lignin, lign in-polysaccharide interactions and association-dissociation kinetics affect biomass deconstruction and thereby cost-effective biofuels production.
Diazonium-functionalized horseradish peroxidase immobilized via addressable electrodeposition: direct electron transfer and electrochemical detection.
TLDR
The direct electrically addressable deposition of diazonium-modified enzymes is examined for electrochemical sensor applications and studies of direct electron transfer of enzymes can benefit from protein electrodes prepared by this method.
Volatile Metabolites Emission by In Vivo Microalgae—An Overlooked Opportunity?
TLDR
VOC emissions from in vivo microalgae; tools and techniques for the collection, storage, transport, detection, and pattern analysis of VOC emissions; linking certain VOCs to biosynthetic/metabolic pathways; and the role of V OCs inmicroalgae growth, infochemical activities, predator-prey interactions, and general SoH are summarized.
Rational Redesign of Glucose Oxidase for Improved Catalytic Function and Stability
TLDR
Four mutants were identified with significantly improved catalytic activity and thermal stability over the parental strain of A. niger GOx and three other mutant enzymes were also identified that had improvements in overall catalysis.
Selective immobilization of DNA and antibody probes on electrode arrays: simultaneous electrochemical detection of DNA and protein on a single platform.
TLDR
Simultaneous electrochemical detection of a DNA sequence related to the breast cancer BRCA1 gene and the human cytokine protein interleukin-12, which is a substantial component in the immune system response and attack of tumor cells, is reported.
Multifunctional Electrode Arrays: Towards a Universal Detection Platform
In this article we review recent work in our laboratory towards the realization of a multianalyte microelectrode detection platform capable of discriminating chemicals and different biomolecules
Encapsulation of S. cerevisiae in Poly(glycerol) Silicate Derived Matrices: Effect of Matrix Additives and Cell Metabolic Phase on Long-Term Viability and Rate of Gene Expression
TLDR
It is found that addition of media components to the glycerol-silica matrix adversely impacted long-term viability in all cases studied, with a 1.3, 1.4, or 5.4 fold decrease in viability after only 9 days of storage in matrices containing yeast pepton...
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