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Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA
TLDR
The DGGE analysis of environmental samples collected from Norway spruce stumps showed that the analysis of fungal DNA extracted directly from wood was usually in accordance with the investigation of mycelial cultures isolated from the same decay column, however, disagreement was observed, which suggests that these two fundamentally different techniques present different views about fungal diversity.
Biogeographical patterns and determinants of invasion by forest pathogens in Europe.
TLDR
A large database of invasive forest pathogens was developed to investigate the patterns and determinants of invasion in Europe, finding thatEradication seems impossible, and prevention seems the only reliable measure, although this will be difficult in the face of global mobility.
Random amplified microsatellites (RAMS) — a novel method for characterizing genetic variation within fungi
TLDR
A novel method, Random Amplified Microsatellites (RAMS), was applied to generate DNA markers in a variety of fungi, based on the polymerase chain reaction (PCR), and uses primers containing microsatellite sequences and degenerate anchors at the 5' end.
RNA reveals a succession of active fungi during the decay of Norway spruce logs
TLDR
This work investigated the changing community of metabolically active fungi during the decomposition of fallen Picea abies logs by directly extracting and barcode sequencing precursor rRNA and compared rRNA-derived amplicons of the 18S and ITS regions in 21 isolates.
Genetic variation in Phlebiopsis gigantea as detected with random amplified microsatellite (RAMS) markers
TLDR
The high degree of polymorphism and heterozygosity observed in RAMS markers suggests that the amount of genetic variation within this fungus is considerable and supports the idea of P. gigantea being a true biological species consisting of a single intersterility group.
Investigations on Heterobasidion annosum s.lat. in central and eastern Asia with the aid of mating tests and DNA fingerprinting
TLDR
To investigate the taxonomy of Heterobasidion in Eurasia, 49 specimens belonging to H. annosum sensu lato from Asia were identified with the aid of mating tests and showed a high degree of compatibility with H. parviporum and H. abietinum.
Widespread Phytophthora infestations in European nurseries put forest, semi-natural and horticultural ecosystems at high risk of Phytophthora diseases
TLDR
At least 47 of the 68 Phytophthora species/taxa detected in nurseries and plantings were exotic species several of which are considered well established in both nurseries or plantings in Europe.
Description of Four Pentachlorophenol-Degrading Bacterial Strains as Sphingomonas chlorophenolica sp. nov.
TLDR
On the basis of chemotaxonomic data, biochemical and physiological properties, and whole cell protein profiles, the four pentachlorophenol-degrading strains Arthrobacter sp.
Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs.
TLDR
Quantification of viral double- and single-stranded RNA with quantitative PCR indicated that co-infecting viral species and viruses with segmented genomes can be detected with small RNA deep sequencing despite vast differences in the amount of RNA.
A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities.
TLDR
Findings suggest that GaBRV-XL represents a novel virus group related to endornaviruses, which is distinct from a broad range of viral taxa but most closely related to Discula destructiva virus 3.
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