• Publications
  • Influence
Introducing DOTUR, a Computer Program for Defining Operational Taxonomic Units and Estimating Species Richness
A computer program, DOTUR, is developed, which assigns sequences to OTUs by using either the furthest, average, or nearest neighbor algorithm for each distance level, which addresses the challenge of assigning sequences to operational taxonomic units (OTUs) based on the genetic distances between sequences. Expand
Metagenomics: Application of Genomics to Uncultured Microorganisms
  • J. Handelsman
  • Medicine, Biology
  • Microbiology and Molecular Biology Reviews
  • 1 December 2004
Reassembly of multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and microheterogeneity, and lateral gene transfer among members of an uncultured community. Expand
Call of the wild: antibiotic resistance genes in natural environments
The presence and spread of antibiotic resistance in non-agricultural, non-clinical environments is explored and the need for more intensive investigation on this subject is demonstrated. Expand
Science faculty’s subtle gender biases favor male students
In a randomized double-blind study, science faculty from research-intensive universities rated the application materials of a student as significantly more competent and hireable than the (identical) female applicant, and preexisting subtle bias against women played a moderating role. Expand
Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products.
The concept of cloning the metagenome to access the collective genomes and the biosynthetic machinery of soil microflora is explored here. Expand
Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms
Phylogenetic analysis of 16S rRNA gene sequences recovered from one of the libraries indicates that the BAC libraries contain DNA from a wide diversity of microbial phyla, including sequences from diverse taxa such as the low-G+C, gram-positive Acidobacterium,Cytophagales, and Proteobacteria. Expand
Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods
The results provide the first comprehensive description of the microbial diversity of a lepidopteran midgut and demonstrate that the plant species in the diet influences the composition of the gut bacterial community. Expand
Introducing SONS, a Tool for Operational Taxonomic Unit-Based Comparisons of Microbial Community Memberships and Structures
A computer program is presented, SONS, which implements nonparametric estimators for the fraction and richness of OTUs shared between two communities. Expand
Fundamentals of Microbial Community Resistance and Resilience
An overview of the concepts of stability that are relevant for microbial communities is provided, and thoughts about the unique insights that systems perspectives – informed by meta-omics data – may provide about microbial community stability are concluded. Expand
Integration of Microbial Ecology and Statistics: a Test To Compare Gene Libraries
Using ∫-LIBSHUFF, a computer program which calculates the integral form of the Cramér-von Mises statistic, it was found that previously published 16S rRNA gene libraries constructed from Scottish and Wisconsin soils contained different bacterial lineages. Expand