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Multiple virus infections in the honey bee and genome divergence of honey bee viruses.
Using uniplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including black queen cell virus (BQCV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbroodExpand
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Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European-like) US isolate
Summary.Three strains of Pepino mosaic virus (PepMV) found in the US have been cloned and sequenced by RT-PCR using total RNA from infected tissue as template, and degenerate potexvirus- and PepMVExpand
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Expression of the Rabies Virus Glycoprotein in Transgenic Tomatoes
We have engineered tomato plants (Lycopersicon esculentumMill var. UC82b) to express a gene for the glycoprotein (G-protein), which coats the outer surface of the rabies virus. The recombinantExpand
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Triple Gene Block Protein Interactions Involved in Movement of Barley Stripe Mosaic Virus
ABSTRACT Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We haveExpand
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Epidemiological risks from mixed virus infections and transgenic plants expressing viral genes.
Publisher Summary This chapter focuses on the epidemiological risks from mixed virus infections and transgenic plants expressing viral genes. Several types of interaction may occur between a virusExpand
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Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies.
A panel of monoclonal antibodies (MAbs) generated against an admixture of 12 potyvirus isolates was used to compare and differentiate diverse potyviruses. Both native and denatured virions of strainsExpand
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Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins
ABSTRACT Barley stripe mosaic virus (BSMV) spreads from cell to cell through the coordinated actions of three triple gene block (TGB) proteins (TGB1, TGB2, and TGB3) arranged in overlapping openExpand
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Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene.
The sequence of 1015 nucleotides from the 3' poly(A) tract of the potyvirus bean yellow mosaic virus (BYMV) RNA has been determined from two cDNA clones. This sequence contained a single long openExpand
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An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue.
Twelve different potyviruses and cytoplasmic inclusion proteins were purified from a range of plant species utilizing a single purification protocol. Highly purified preparations have been obtainedExpand
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Use of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweetpotato
Sweetpotato is susceptible to infection by several filamentous viruses of which only the aphid-transmitted sweetpotato feathery mottle virus (SPFMV) has been well-characterized. Sweetpotato mildExpand
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