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Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
Visualization of Melanosome Dynamics within Wild-Type and Dilute Melanocytes Suggests a Paradigm for Myosin V Function In Vivo
It is concluded that long-range, bidirectional, microtubule-dependent melanosome movements, coupled with actomyosin Va–dependent capture of melanosomes in the periphery, is the predominant mechanism responsible for the centrifugal transport and peripheral accumulation of melanOSomes in mouse melanocytes.
Identification of an organelle receptor for myosin-Va
The results provide the first molecular description of an organelle receptor for an actin-based motor, illustrate how alternate exon usage can be used to specify cargo, and further expand the functional repertoire of Rab GTPases and their effectors.
Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells
Actin and myosin IIA have been implicated in the inward movement of receptor clusters at the immunological synapse of T lymphocytes. This study defines their spatial organization and quantifies their
Two-headed binding of a processive myosin to F-actin
Myosins are motor proteins in cells. They move along actin by changing shape after making stereospecific interactions with the actin subunits. As these are arranged helically, a succession of steps
Mutation of melanosome protein RAB38 in chocolate mice
Examination of similarities in human gene expression patterns by using microarray analysis reveals that RAB38, a small GTP binding protein, demonstrates a similar expression profile to melanocytic genes, and the fact that green fluorescent protein-tagged RAB 38 colocalizes with end-stage melanosomes in wild-type melanocytes, suggest that Rab38 plays a role in the sorting of TYRP1.
Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes
Rab10 activation promotes GLUT4 storage vesicle recruitment to the plasma membrane in response to insulin and coordinates with myosin-Va to mediate vesicle fusion.
Defective Granule Exocytosis in Rab27a-Deficient Lymphocytes from Ashen Mice
It is concluded that Rab27a is required for a late step in granule exocytosis, compatible with current models of Rab protein function in vesicle docking and fusion.
Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics
Two approaches are used to improve the resolution of SIM to allow live cell imaging of dynamic cellular processes, including endocytosis and cytoskeleton remodeling, and it is demonstrated that the resolution for these methods is comparable with or better than other SR approaches yet allowed us to image at far higher speeds, and for far longer durations.
Walking to work: roles for class V myosins as cargo transporters
There is increasing evidence in vertebrate cells that class V myosins not only tether organelles to actin but also can serve as short-range, point-to-point organelle transporters, usually following long- range, microtubule-dependent organelle transport.