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Citrus tristeza virus: a pathogen that changed the course of the citrus industry.
Analysis of genetic variation in CTV isolates revealed conservation of genomes in distant geographical regions, with a limited repertoire of genotypes, uneven distribution of variation along the gRNA, (3) frequent recombination events and (4) different selection pressures shaping CTV populations. Expand
Genetic Variation of Citrus Tristeza VirusIsolates from California and Spain: Evidence for Mixed Infections and Recombination
Examination of the population structure and genetic variation of four genomic regions within and between 30 Citrus tristeza virus isolates from Spain and California suggested that the genomes of some CTV sequence variants originated by recombination events between diverged sequence variants. Expand
Sequences of Citrus Tristeza VirusSeparated in Time and Space Are Essentially Identical
ABSTRACT The first Citrus tristeza virus (CTV) genomes completely sequenced (19.3-kb positive-sense RNA), from four biologically distinct isolates, are unexpectedly divergent in nucleotide sequenceExpand
The complete genome sequence of the major component of a mild citrus tristeza virus isolate.
The genome of the Spanish mild isolate T385 of citrus tristeza virus was completely sequenced and compared with the genomes of the severe isolates T36, VT and SY568, suggesting that the central region in the SY568 genome results from RNA recombination between two CTV genomes, one of which was almost identical to T385. Expand
Polymorphism of the 5′ terminal region of Citrus tristeza virus (CTV) RNA: Incidence of three sequence types in isolates of different origin and pathogenicity
None of the sequence types could be traced to a precise geographic area, as all types were detected in isolates from at least nine of the 12 countries from which samples were taken. Expand
Differentiation of citrus tristeza closterovirus (CTV) isolates by single‐strand conformation polymorphism analysis of the coat protein gene
SSCP analysis may provide a procedure to identify and differentiate CTV isolates based on comparisons of several genes or gene regions because it is rapid and cheap and may drastically reduce the amount of sequencing necessary for accurate comparisons. Expand
Pathogen-derived resistance to Citrus tristeza virus (CTV) in transgenic mexican lime (Citrus aurantifolia (Christ.) Swing.) plants expressing its p25 coat protein gene
Protection was efficient against non-homologousCTV strains and was generally accompanied by high accumulation of p25 CP in theprotected lines, which suggest a CP-mediated protection mechanism in most cases. Expand
Efficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus
The coat protein gene of citrus tristeza virus has been introduced into Mexican lime (Citrus aurantifolia Swing.) plants by using an improved Agrobacterium-mediated genetic transformation system and expression of the CTV-CP transgene was demonstrated. Expand
Molecular variability of the 5'- and 3'-terminal regions of citrus tristeza virus RNA.
The presence of a conserved putative "zinc-finger" domain adjacent to a basic region in p23, the predicted product of ORF 11, suggests that this protein might act as a regulatory factor during virus replication. Expand
New defective RNAs from citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation.
Two of the CTV isolates that differed greatly in their pathogenicity contained an identical D- RNA, suggesting that it is unlikely that this D-RNA is involved in symptom modulation, which may be caused by another factor. Expand