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Guidelines for the use and interpretation of assays for monitoring autophagy
TLDR
These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro
TLDR
The in vitro scratch assay is particularly suitable for studies on the effects of cell–matrix and cell–cell interactions on cell migration, mimic cell migration during wound healing in vivo and are compatible with imaging of live cells during migration to monitor intracellular events if desired.
Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy.
TLDR
A novel mammalian autophagy factor, Atg13, is reported, which forms a stable approximately 3-MDa protein complex with ULK1 and FIP200, and suggests that mTORC1 suppressesAutophagy through direct regulation of the approximately 3,MDa ULK 1-Atg13-FIP200 complex.
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells
TLDR
The results suggest that FIP200 is a novel mammalian autophagy factor that functions together with ULKs, which regulates diverse cellular functions such as cell size, proliferation, and migration.
Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins
TLDR
The molecular requirements for LAP are characterized, and Rubicon is identified as being required for L AP but not autophagy, thereby elucidating the importance of LAP in response to A. fumigatus infection.
Recognition of RNA N6-methyladenosine by IGF2BP Proteins Enhances mRNA Stability and Translation
TLDR
This work reports the insulin-like growth factor 2 mRNA-binding proteins as a distinct family of m6A readers that target thousands of mRNA transcripts through recognizing the consensus GG(m6A)C sequence, and identifies IGF2BPs as an additional class of N6-methyladenosine (m 6A) reader proteins.
CXCR1 blockade selectively targets human breast cancer stem cells in vitro and in xenografts.
TLDR
The development of a strategy to target these breast cancer stem cells (CSCs) through blockade of the IL-8 receptor CXCR1 and repertaxin was able to specifically target the CSC population in human breast cancer xenografts, retarding tumor growth and reducing metastasis.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Phosphorylation of Tyrosine 397 in Focal Adhesion Kinase Is Required for Binding Phosphatidylinositol 3-Kinase*
TLDR
It is suggested that FAK activation and autophosphorylation at Tyr-397 may lead to its association with PI 3-kinase through the SH2 domains of p85, which can subsequently activate PI 3 -kinase during cell adhesion.
Signal transduction by focal adhesion kinase in cancer
  • Jihe Zhao, J. Guan
  • Biology, Chemistry
    Cancer and Metastasis Reviews
  • 24 January 2009
TLDR
The intensive research on FAK signaling in cancer have yielded a wealth of information on this pivotal kinase and these and future studies are leading to potentially novel therapies for cancer.
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