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Guidelines for the use and interpretation of assays for monitoring autophagy
TLDR
These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. Expand
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms. Expand
Potential therapeutic applications of autophagy
TLDR
A better understanding of autophagy is needed to allow its manipulation for therapeutic purposes, and new insights into the molecular mechanisms are now leading to the discovery of exciting new potential drug targets. Expand
Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate
TLDR
An experimental structure for wild-type, full-length DnaK, complexed with the peptide NRLLLTG and with ADP is reported and suggests that the SBD collides with the NBD at this area to establish allosteric communication. Expand
A Field of Myocardial-Endocardial NFAT Signaling Underlies Heart Valve Morphogenesis
TLDR
It is shown that the initiation of heart valve morphogenesis in mice requires calcineurin/NFAT to repress VEGF expression in the myocardium underlying the site of prospective valve formation, and this mechanism also operates in zebrafish, indicating a conserved role for calcineURin/ NFAT signaling in vertebrate heart valve Morphogenesis. Expand
Heat Shock Proteins 70 and 90 Inhibit Early Stages of Amyloid β-(1–42) Aggregation in Vitro*
TLDR
The results suggest that if these chaperones are present in the same cellular compartment in which Aβ is produced, Hsp70/40 and Hsp90 may suppress the early stages of self-assembly, consistent with a model in which pharmacological activation of chaperone might have a favorable therapeutic effect on Alzheimer disease. Expand
Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP.
TLDR
The results suggest that functional pairing of E3s with ataxin-3 or similar DUBs represents an important point of regulation in ubiquitin-dependent protein quality control and shed light on disease pathogenesis in SCA3, a neurodegenerative disorder caused by polyglutamine expansion in ataxIn-3. Expand
Heat shock protein 70 (hsp70) as an emerging drug target.
TLDR
In the ER and mitochondria, the Hsp70 family members are thought to fulfill specific functions and have unique substrates, with BiP playing key roles in the folding and quality control of ER proteins and mtHsp70 being involved in the import and export of proteins from the mitochondria. Expand
Allostery in the Hsp70 chaperone proteins.
TLDR
X-ray crystallography, NMR spectroscopy, and other biophysical techniques have contributed much to the understanding of the allosteric mechanism linking these activities and the effect of co-chaperones on this mechanism. Expand
Targeting Hsp90/Hsp70-based protein quality control for treatment of adult onset neurodegenerative diseases.
TLDR
How drugs that modulate proteostasis by inhibiting Hsp90 function or promoting Hsp70 function enhance the degradation of the critical aggregating proteins and ameliorate toxic symptoms in cell and animal disease models is discussed. Expand
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