• Publications
  • Influence
D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport
The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-Affinity transport system for D- ribose is present in Escherichia coli K-12. Expand
Preparative enzymatic synthesis and hydrophobic chromatography of acyl-acyl carrier protein.
  • C. Rock, J. Garwin
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 10 August 1979
The high resolution obtained using 2-propanol gradients to separate acyl-ACP species suggests that similar techniques would be applicable to the chromatography of protein mixtures on hydrophobic supports. Expand
Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis.
Cvc- mutants of Escherichia coli are deficient in the synthesis of cis-vaccenic acid and in the temperature control of fatty acid synthesis. In this communication, it is demonstrated that theseExpand
Efficacy and safety study of 1.62% testosterone gel for the treatment of hypogonadal men.
Treatment with 1.62% testosterone gel was safe and efficacious, resulting in an acceptable percentage of hypogonadal males achieving eugonadal serum testosterone levels, according to a multicenter, randomized, double-blind, placebo-controlled study. Expand
Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli.
The properties of synthase II are consistent with the proposed role of this enzyme in the modulation of fatty acid synthesis by temperature using five long chain acyl-acyl carrier protein substrates. Expand
Purification and partial sequence analysis of a 37-kDa protein that inhibits phospholipase A2 activity from rat peritoneal exudates.
Properties of the molecule suggest that it is a member of a family of steroid-induced anti-inflammatory proteins collectively referred to as lipocortin, and it is inferred that the inhibitor is highly conserved evolutionarily. Expand
Genetic and Biochemical Analyses of Escherichia coli Mutants Altered in the Temperature-Dependent Regulation of Membrane Lipid Composition
The Vtr and fabFl mutations show very tight genetic linkage, indicating intra-allelic complementation, and the synthase II of Vtr strains is abnormally sensitive to cerulenin, an antibiotic that specifically inhibits synthases I and II. Expand
Insulin selectively slows the degradation rate of tyrosine aminotransferase.
It is concluded that insulin induces tyrosine aminotransferase in rat hepatoma cells primarily by selectively slowing the rate of degradation of the enzyme. Expand
Overproduction of cis-Vaccenic Acid and Altered Temperature Control of Fatty Acid Synthesis in a Mutant of Escherichia coli
A mutant of Escherichia coli has been characterized which overproduces cis-vaccenic acid in the temperature range of 30 to 42°C. The mutational lesion acts within the fatty acid biosynthetic pathwayExpand
Preparative enzymatic synthesis of acyl-acyl carrier protein.
It is found that when large amounts of acyl-ACP are being prepared it is useful to recover acel-ACP synthetase from the incubation mixtures for use in further syntheses, and the presence of the acyl moiety on ACP also stabilizes the protein to pH-induced hydrodynamic expansion, permitting the separation of ACP from acyl -ACP on gel filtration columns. Expand