Hyaluronan-mediated angiogenesis in vascular disease: uncovering RHAMM and CD44 receptor signaling pathways.
Oligosaccharides of hyaluronan induce angiogenesis through distinct CD44 and RHAMM-mediated signalling pathways involving Cdc2 and gamma-adducin.
- S. Matou-Nasri, J. Gaffney, S. Kumar, M. Slevin
- Biology, ChemistryInternational Journal of Oncology
- 1 October 2009
The data presented here extend the understanding of key stages of the processes of o-HA-induced angiogenesis which may have relevance to tumour progression.
Angiogenic Oligosaccharides of Hyaluronan Induce Multiple Signaling Pathways Affecting Vascular Endothelial Cell Mitogenic and Wound Healing Responses*
It is shown that o-HA-induced bovine aortic endothelial cell proliferation, wound recovery, and ERK1/2 activation were also partially dependent on Ras activation, and that o.HA-stimulated tyrosine phosphorylation of the adapter protein Shc, as well as its association with Sos1, suggested a possible role for Src in activation of PLCγ1 and interaction between two distinct o- HA-induced signaling pathways.
Angiogenic oligosaccharides of hyaluronan induce protein tyrosine kinase activity in endothelial cells and activate a cytoplasmic signal transduction pathway resulting in proliferation.
- M. Slevin, J. Krupiński, S. Kumar, J. Gaffney
- BiologyLaboratory investigation; a journal of technical…
- 1 August 1998
Phosphorylation of the CD44 receptor results in an increase in tyrosine phosphorylation, leading to the activation of a cytoplasmic cascade and cell proliferation; this concurs with previous work, which showed that o-HA-induced proliferation of endothelial cells is CD44-receptor-mediated and accompanied by early response gene activation.
Serial measurement of vascular endothelial growth factor and transforming growth factor-beta1 in serum of patients with acute ischemic stroke.
- M. Slevin, J. Krupiński, A. Słowik, P. Kumar, A. Szczudlik, J. Gaffney
- Medicine, BiologyStroke
- 1 August 2000
VEGF may play an important role in the pathophysiology of acute ischemic stroke and could be of value in future treatment strategies and correlation between stroke severity and VEGF concentration suggests it could be involved in the subsequent repair processes resulting in partial recovery after stroke.
Changes in hyaluronan production and metabolism following ischaemic stroke in man.
- Ahmed Al'Qteishat, J. Gaffney, M. Slevin
- Biology, MedicineBrain : a journal of neurology
- 1 August 2006
It is found that the production of total HA and low molecular mass 3-10 disaccharides of HA (o-HA) was increased in post-mortem tissue and in the serum of patients 1, 3, 7 and 14 days after ischaemic stroke.
Aminosalicylic acid reduces the antiproliferative effect of hyperglycaemia, advanced glycation endproducts and glycated basic fibroblast growth factor in cultured bovine aortic endothelial cells:…
- Y. Duraisamy, J. Gaffney, M. Slevin, Christopher A. Smith, Kenneth Williamson, N. Ahmed
- Biology, MedicineMolecular and Cellular Biochemistry
- 1 April 2003
Compounds like aminoguanidine and, in particular, aminosalicylic acid protect endothelial cells against glucose-mediated toxicity and may therefore have therapeutic potential.
A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion
The findings confirmed previous studies reporting that gene expression screening can detect known and unknown transcriptional features of stroke and highlight the importance of research using human brain tissue in the search for novel therapeutic agents.
Therapeutic applications of hyaluronan.
- J. Gaffney, S. Matou-Nasri, M. Grau-Olivares, M. Slevin
- Biology, ChemistryMolecular Biosystems
- 17 February 2010
Some of the recent therapeutic applications of hyaluronan in tissue repair, as a drug delivery system and the synthesis, application and delivery of hyAluronan nanoparticles to target drugs to sites of disease are discussed.
Differences in the uptake of modified low density lipoproteins by tissue cultured endothelial cells.
A quantitative examination of lipoprotein uptake by flow cytometry showed cells in G2M took up 45% more Ac-LDL than those in G1S, indicating that the Ac- LDL receptor is not down-regulated to the same extent as that for LDL.