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Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ
The asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome is determined, revealing that in situ, the viral RNA genome can adopt a defined conformation. Expand
MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps.
A fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes), which is computationally more rapid than existing methods in the field and offers the option of local filtering of the original map based on the calculated local resolution. Expand
Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy.
Scipion is a software framework for integrating several 3DEM software packages through a workflow-based approach that allows the execution of reusable, standardized, traceable and reproducible image-processing protocols. Expand
Epitope Insertion at the N-Terminal Molecular Switch of the Rabbit Hemorrhagic Disease Virus T=3 Capsid Protein Leads to Larger T=4 Capsids
The calicivirus capsid protein, and perhaps that of many other viruses, thus can encode polymorphism modulators that are not anticipated from the plane sequence, with important implications for understanding virus assembly and evolution. Expand
A review of resolution measures and related aspects in 3D Electron Microscopy.
This paper provides theoretical justifications for some common practices in the field for which a formal justification was missing and reviews the consequences for the number of particles needed to achieve a certain resolution and how to analyze the Signal-to-Noise Ratio for a sequence of imaging operations. Expand
Structural and functional insights into Escherichia coli α2-macroglobulin endopeptidase snap-trap inhibition
Significance Proteolytic enzymes are inhibited in vivo by protein inhibitors. Such inhibitors are used by symbiotic bacteria in our gut to protect themselves from digestive peptidases. This is theExpand
Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses
The results indicate that the intimate virus-fungus partnership has altered the capsid genome-protective and/or receptor-binding functions in Rosellinia necatrix quadrivirus 1, indicating that Fungal virus evolution has tended to allocate enzyme activities to the virus capsid outer surface. Expand
Structural Basis for the Development of Avian Virus Capsids That Display Influenza Virus Proteins and Induce Protective Immunity
It is reported here that the capsid of a major avian pathogen, infectious bursal disease virus (IBDV), can accommodate heterologous proteins to induce protective immunity and establish the basis for a new generation of multivalent IBDV-based vaccines. Expand
Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit
Era’s role in ribosome assembly is established and new roles in maintaining ribosomal homeostasis are suggested, including binding of Era to the mature 30S subunit and the decoding center preventing binding of YjeQ, another assembly factor. Expand
Near Atomic Structure of an Atadenovirus Reveals a Conserved Capsid-Binding Motif and Intergenera Variations in Cementing Proteins
This work shows how the network of minor coat proteins differs between AdV genera and relates to virus evolution and capsid stability properties. Expand