J. Fletcher

Publications117
h-index25
Citations2,159
Highly Influential Citations119
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The phytopathogenic mollicute-insect vector interface: a closer look.
TLDR
Adhesion of spiroplasmas to cultured leafhopper cells was inhibited by proteases, suggesting that adherence to host cells is mediated by spiroPLasma membrane protein(s).
The Major Antigenic Membrane Protein of “Candidatus Phytoplasma asteris” Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors
TLDR
The vector-specific interaction between phytoplasma Amp and insect ATP synthase is demonstrated for the first time, and this work supports the hypothesis that host actin is involved in the internalization and intracellular motility of phy toplasmas within their vectors.
Effect of route of introduction and host cultivar on the colonization, internalization, and movement of the human pathogen Escherichia coli O157:H7 in spinach.
TLDR
It is suggested that the spinach phylloplane is a supportive niche for E. coli O157:H7, but no conclusive evidence was found for natural entry into the plant interior, which is relevant for interventions aimed at minimizing produce contamination by human pathogens.
Characterization of Spiroplasma citri adhesion related protein SARP1, which contains a domain of a novel family designated sarpin.
Plant Pathogen Forensics: Capabilities, Needs, and Recommendations
TLDR
This work assesses currently available information, technologies, and resources that were developed originally to ensure plant health but also could be utilized for postintroduction plant pathogen forensics and recommendations for prioritization of efforts and resource expenditures are presented.
Spiroplasma citri Movement into the Intestines and Salivary Glands of Its Leafhopper Vector, Circulifer tenellus.
TLDR
Cytopathological effects of spiroplasma infection in salivary cells included loss of membrane and basal lamina integrity, presence in some cells of irregular inclusion-like structures containing dense matrices of filamentous material that labeled with anti S. citri antibodies, and apparent disorganization of the endoplasmic reticulum.
Association of Escherichia coli O157:H7 with filth flies (Muscidae and Calliphoridae) captured in leafy greens fields and experimental transmission of E. coli O157:H7 to spinach leaves by house flies…
TLDR
Results indicate that flies are capable of contaminating leafy greens under experimental conditions and confirm the importance of further investigation of the role of insects in contamination of fresh produce.
Spiroplasma citri Surface Protein P89 Implicated in Adhesion to Cells of the Vector Circulifer tenellus.
TLDR
Results suggest that a spiroplasma surface protein, P89, has a role in S. citri adherence to C. tenellus cells.
Validation of high throughput sequencing and microbial forensics applications
TLDR
Criteria that should be considered for HTS validation for microbial forensics are presented here and comprise a foundation for investigators to establish, validate and implement HTS as a tool in microbialForensics, enhancing public safety and national security.
Serratia marcescens, a Phloem-Colonizing, Squash Bug -Transmitted Bacterium: Causal Agent of Cucurbit Yellow Vine Disease.
TLDR
The experiments represent a completion of the steps of Koch's postulates, demonstrating that S. marcescens is the causal agent of CYVD and that the squash bug, A. tristis, is a vector of the pathogen.
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