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Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function.
TLDR
The solution structure of this essential pseudoknot, presented here, reveals an extended triple helix surrounding the helical junction that is required for telomerase activity. Expand
Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution.
TLDR
This aptamer structure was determined by NMR and illustrates that this molecule forms a specific folded structure, which may be used in the further development of oligonucleotide-based thrombin inhibitors. Expand
New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes
TLDR
A new approach to facilitate assignment of RNA spectra using a suite of four 2D-filtered/edited NOESY experiments in combination with base-type-specific isotopically labeled RNA to facilitate NOE assignment of larger RNAs and an RNA-protein complex is presented. Expand
Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III.
TLDR
Results show how a single dsRBD can convey specificity for particular RNA targets, by structure specific recognition of a conserved tetraloop fold. Expand
The selectivity for K+ versus Na+ in DNA quadruplexes is dominated by relative free energies of hydration: a thermodynamic analysis by 1H NMR.
TLDR
Analysis of competition between Na+ and K+ for coordination by G quartets using the oligonucleotide d(G3T4G3) as a model system indicates that at least two K+ are coordinated by the three quartets of the dimeric molecule, and perfect fits of the data are obtained for two Na+ being displaced by two K+. Expand
Solution structures of UBA domains reveal a conserved hydrophobic surface for protein-protein interactions.
TLDR
Comparison of the structures of the two UBA domains indicates that the binding site of HIV-1 Vpr on UBA(2) does not completely overlap the ubiquitin binding site, and predicts that this hydrophobic surface patch is a common protein-interacting surface present in diverse UBA domain. Expand
Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA.
TLDR
The solution structure of the preQ(1) riboswitch aptamer domain from Bacillus subtilis bound to preZ(1), which is a unique compact pseudoknot with three loops and two stems that encapsulates preQ (1) at the junction between the two stems, is reported. Expand
DNA A-tract bending in three dimensions: solving the dA4T4 vs. dT4A4 conundrum.
TLDR
Results of gel electrophoretic mobility studies are consistent with the higher-order structure of the DNA and furthermore depend on the nature of the monovalent cation present in the running buffer. Expand
The S. cerevisiae architectural HMGB protein NHP6A complexed with DNA: DNA and protein conformational changes upon binding.
TLDR
The refined solution structures of the NHP6A-DNA complex as well as the free 15bp DNA are presented to provide insight into the factors that contribute to binding site selection and DNA deformations in the complex. Expand
Solution structure of protegrin-1, a broad-spectrum antimicrobial peptide from porcine leukocytes.
TLDR
The structure of PG-1 suggests ways in which the peptide may interact with itself or other molecules to form the membrane pores and the large membrane-associated assemblages observed in protegrin-treated, gram-negative bacteria. Expand
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