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Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo
Normal cells can permanently lose the ability to proliferate when challenged by potentially oncogenic stress, a process termed cellular senescence. Senescence-associated beta-galactosidase (SA-βgal)Expand
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Enhanced Peroxynitrite Formation Is Associated with Vascular Aging
Vascular aging is mainly characterized by endothelial dysfunction. We found decreased free nitric oxide (NO) levels in aged rat aortas, in conjunction with a sevenfold higher expression and activityExpand
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Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells.
Senescence-associated (beta)-galactosidase is widely used as a biomarker of replicative senescence. However, it remains unknown whether this is a distinct enzyme active at pH 6, and differentiallyExpand
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Vascular endothelial senescence: from mechanisms to pathophysiology.
Most mitotically competent mammalian cell types can react to stress by undergoing a phenotypically distinctive and permanent form of growth arrest called "cellular senescence." This response has beenExpand
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Does nitric oxide modulate mitochondrial energy generation and apoptosis?
The physiological role of nitric oxide (NO) in the maintenance of vascular tone, in synaptic transmission and in cellular defence is now firmly established. Recent evidence indicates that NO can alsoExpand
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Chronic oxidative stress compromises telomere integrity and accelerates the onset of senescence in human endothelial cells
Replicative senescence and oxidative stress have been implicated in ageing, endothelial dysfunction and atherosclerosis. Replicative senescence is determined primarily by telomere integrity. InExpand
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Educational attainment but not measures of current socioeconomic circumstances are associated with leukocyte telomere length in healthy older men and women
Low socioeconomic status (SES) may be associated with accelerated biological aging, but findings relating SES with telomere length have been inconsistent. We tested the hypotheses that shorterExpand
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Senescence-associated β-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells
Acid β-D-galactosidase (EC 3.2.1.23) is a eukaryotic hydrolase localized in the lysosome (reviewed in Suzuki et al., 1995). It cleaves β-linked terminal galactosyl residues from a wide range ofExpand
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Nitric oxide and mitochondrial signaling: from physiology to pathophysiology.
Nitric oxide (NO) has been known for many years to bind to cytochrome C oxidase, the terminal acceptor in the mitochondrial electron transport chain, in competition with oxygen. This interaction mayExpand
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Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes.
OBJECTIVE Maintenance of the mitochondrial membrane potential (Deltapsim) is fundamental for the normal performance and survival of cells such as cardiomyocytes, that have a high energy requirement.Expand
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