Molecular Bases for Circadian Clocks
- J. Dunlap
- 22 January 1999
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
- H. Colot, Gyungsoon Park, J. Dunlap
- BiologyProceedings of the National Academy of Sciences
- 5 July 2006
This study describes a method for rapidly creating knockout mutants in which it makes use of yeast recombinational cloning, Neurospora mutant strains deficient in nonhomologous end-joining DNA repair, custom-written software tools, and robotics.
The genome sequence of the filamentous fungus Neurospora crassa
A high-quality draft sequence of the N. crassa genome is reported, suggesting that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes.
Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism
An analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa reveals potential new targets for antifungals as well as loci implicated in human and plant physiology and disease.
Neurospora wc-1 and wc-2: transcription, photoresponses, and the origins of circadian rhythmicity.
DNA binding and transcriptional activation can now be associated with a clock gene that may provide a positive element in the feedback loop, and similarities between the PAS-domain regions of molecules involved in light perception and circadian rhythmicity in several organisms suggest an evolutionary link between ancient photoreceptor proteins and more modern proteins required for circadian oscillation.
Light-Induced Resetting of a Mammalian Circadian Clock Is Associated with Rapid Induction of the mPer1 Transcript
White Collar-1, a Circadian Blue Light Photoreceptor, Binding to the frequency Promoter
It is confirmed that WC-1, with flavin adenine dinucleotide as a cofactor, is the blue light photoreceptor that mediates light input to the circadian system through direct binding (with WC-2) to the frq promoter.
The PAS Protein VIVID Defines a Clock-Associated Feedback Loop that Represses Light Input, Modulates Gating, and Regulates Clock Resetting
Interconnected feedback loops in the Neurospora circadian system.
In Neurospora crassa, white collar 1 (WC-1), a transcriptional activator and positive clock element, is rhythmically expressed from a nonrhythmic steady-state pool of wc-1 transcript, consistent with…