Simultaneous quantitative profiling of N-acyl-l-homoserine lactone and 2-alkyl-4(1H)-quinolone families of quorum-sensing signaling molecules using LC-MS/MS
Application of the LC-MS/MS method showed significant effects of growth medium type on the ratio and the nature of the QSSMs synthesized and the dramatic effect of single, double and triple mutations in the P. aeruginosa QS synthase genes.
Quorum sensing by 2-alkyl-4-quinolones in Pseudomonas aeruginosa and other bacterial species.
The most recent findings on the biosynthesis and regulation of PQS and other AQs including the discovery of AQs in other bacterial species are summarized.
Genetic and functional characterization of the gene cluster directing the biosynthesis of putisolvin I and II in Pseudomonas putida strain PCL1445.
Sequence data indicate that the adenylation domain of the 11th module prioritizes the recognition of Val instead of Leu or Ile and consequently favoursPutisolvin I production over putisolvin II, and detailed analysis of the thiolation domains suggests that the first nine modules recognize the d form of the amino acid residues while the last module the l form, indifferently.
The Heat Shock Genes dnaK, dnaJ, and grpE Are Involved in Regulation of Putisolvin Biosynthesis in Pseudomonas putida PCL1445
Analysis of putisolvin production and expression studies indicate that dnaK, together with the dnaJ and grpE heat shock genes, takes part in the positive regulation (directly or indirectly) ofPutisolvin biosynthesis at the transcriptional level.
Transmission of African cassava mosaic geminivirus by the whitefly (Bemisia tabaci).
- J. Dubern
Studies on Variability of Peanut Clump Virus: Symptomatology and Serology
Serogroups do not correlate with the geographic origin and these did not correspond to the five groups formed in an arbitrary classification based on the symptomatology of C. amaranticolor.
Analysis of the sequence diversity of the P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses.
- M. Aleman-Verdaguer, C. Goudou-Urbino, J. Dubern, R. Beachy, C. Fauquet
- BiologyJournal of General Virology
- 1 June 1997
P3 appeared to be conserved between YMV isolates even though this region was variable between potyvirus species, and intraspecies molecular diversity differed from interspecies molecular diversity in the P3, P1 and N-terminal regions.
Differentiation of yam virus isolates using symptomatology, western blot assay, and monoclonal antibodies
There is a significant variability among yam virus isolates which is unrelated to the origin of the isolate (geographic or host species) and it is suggested that precautions should be taken to avoid international exchange of infected material.
Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
The introduction and maintenance of 20 yam species, under slow growth conditions and cryopreservation using the encapsulation/dehydration technique for long-term preservation of yam germplasm were succeeded.
Unravelling the Genome-Wide Contributions of Specific 2-Alkyl-4-Quinolones and PqsE to Quorum Sensing in Pseudomonas aeruginosa
The pqs quorum sensing (QS) system is crucial for Pseudomonas aeruginosa virulence both in vitro and in animal models of infection and is considered an ideal target for the development of…